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Zhang LQ, Wei K, Cheng H, et al. Accumulation of catechins and expression of catechin synthetic genes in Camellia sinensis at different developmental stages. Bot Stud. 2016;57(1):31doi: 10.1186/s40529-016-0143-9.
Zhang, L. Q., Wei, K., Cheng, H., Wang, L. Y., & Zhang, C. C. (2016). Accumulation of catechins and expression of catechin synthetic genes in Camellia sinensis at different developmental stages. Botanical studies, 57(1), 31. https://doi.org/10.1186/s40529-016-0143-9
Zhang, Li-Qun, et al. "Accumulation of catechins and expression of catechin synthetic genes in Camellia sinensis at different developmental stages." Botanical studies vol. 57,1 (2016): 31. doi: https://doi.org/10.1186/s40529-016-0143-9
Zhang LQ, Wei K, Cheng H, Wang LY, Zhang CC. Accumulation of catechins and expression of catechin synthetic genes in Camellia sinensis at different developmental stages. Bot Stud. 2016 Dec;57(1):31. doi: 10.1186/s40529-016-0143-9. Epub 2016 Oct 24. PMID: 28597441; PMCID: PMC5430556.
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Bot Stud. 2016 Dec;57(1):31. doi: 10.1186/s40529-016-0143-9. Epub 2016 Oct 24.

Accumulation of catechins and expression of catechin synthetic genes in Camellia sinensis at different developmental stages.

Botanical studies

Li-Qun Zhang, Kang Wei, Hao Cheng, Li-Yuan Wang, Cheng-Cai Zhang

Affiliations

  1. Key Laboratory of Tea Biology and Resources Utilization, Tea Research Institute Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Ministry of Agriculture, No. 9, Meiling South Road, Xihu District, Hangzhou, 310008, Zhejiang, China.
  2. Key Laboratory of Tea Biology and Resources Utilization, Tea Research Institute Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Ministry of Agriculture, No. 9, Meiling South Road, Xihu District, Hangzhou, 310008, Zhejiang, China. [email protected].

PMID: 28597441 PMCID: PMC5430556 DOI: 10.1186/s40529-016-0143-9

Abstract

BACKGROUND: Catechins are the main polyphenol compounds in tea (Camellia sinensis). To understand the relationship between gene expression and product accumulation, the levels of catechins and relative expressions of key genes in tea leaves of different developmental stages were analyzed.

RESULTS: The amounts of catechins differed significantly in leaves of different stages, except for gallocatechin gallate. Close correlations between the expression of synthesis genes and the accumulation of catechins were identified. Correlation analysis showed that the expressions of chalcone synthase 1, chalcone synthase 3, anthocyanidin reductase 1, anthocyanidin reductase 2 and leucoanthocyanidin reductase genes were significantly and positively correlated with total catechin contents, suggesting their expression may largely affect total catechin accumulation. Anthocyanidin synthase was significantly correlated with catechin. While both ANRs and LAR were significantly and positively correlated with the contents of (-)-epigallocatechin gallate and (-)-epicatechin gallate.

CONCLUSION: Our results suggest synergistic changes between the expression of synthetic genes and the accumulation of catechins. Based on our findings, anthocyanidin synthase may regulate earlier steps in the conversion of catechin, while the anthocyanidin reductase and leucoanthocyanidin reductase genes may both play important roles in the biosynthesis of galloylated catechins.

Keywords: Anthocyanidin reductase; Anthocyanidin synthase; Camellia sinensis; Catechins; Chalcone synthase; Leucoanthocyanidin reductase

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