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Vos AM, Jurak E, Pelkmans JF, et al. H. AMB Express. 2017;7(1):124doi: 10.1186/s13568-017-0424-z.
Vos, A. M., Jurak, E., Pelkmans, J. F., Herman, K., Pels, G., Baars, J. J., Hendrix, E., Kabel, M. A., Lugones, L. G., & Wösten, H. A. B. (2017). H. AMB Express, 7(1), 124. https://doi.org/10.1186/s13568-017-0424-z
Vos, Aurin M, et al. "H." AMB Express vol. 7,1 (2017): 124. doi: https://doi.org/10.1186/s13568-017-0424-z
Vos AM, Jurak E, Pelkmans JF, Herman K, Pels G, Baars JJ, Hendrix E, Kabel MA, Lugones LG, Wösten HAB. H. AMB Express. 2017 Dec;7(1):124. doi: 10.1186/s13568-017-0424-z. Epub 2017 Jun 17. PMID: 28629207; PMCID: PMC5474230.
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AMB Express. 2017 Dec;7(1):124. doi: 10.1186/s13568-017-0424-z. Epub 2017 Jun 17.

H.

AMB Express

Aurin M Vos, Edita Jurak, Jordi F Pelkmans, Koen Herman, Gill Pels, Johan J Baars, Ed Hendrix, Mirjam A Kabel, Luis G Lugones, Han A B Wösten

Affiliations

  1. Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
  2. Laboratory of Food Chemistry, Wageningen University, Bornse Weilanden 9, 6708 WG, Wageningen, The Netherlands.
  3. Plant Breeding, Wageningen University and Research Centre, 6700 AJ, Wageningen, The Netherlands.
  4. Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands. [email protected].

PMID: 28629207 PMCID: PMC5474230 DOI: 10.1186/s13568-017-0424-z

Abstract

Degradation of lignin by fungi enhances availability of cellulose and hemicellulose in plant waste and thereby increases the amount of carbon source available to these microorganisms. The button mushroom Agaricus bisporus degrades only about half of the lignin in compost and about 40% of the carbohydrates remain unutilized during mushroom cultivation. Here it was assessed whether over-expression of the manganese peroxidase gene mnp1 improves lignin degradation and, as a consequence, carbohydrate breakdown by A. bisporus. Transformants expressing mnp1 under the control of actin regulatory sequences produced MnP activity in malt extract medium, while the parental strain A15 did not. MnP activity was increased 0.3- and 3-fold at casing and after the 2nd flush of a semi-commercial cultivation, respectively, when compared to strain A15. Pyrolysis-GC-MS showed that overexpression of MnP decreased phenylmethane and phenylethane type lignin relative to the phenylpropane type after the 2nd flush. However, it neither affected the syringyl/guaiacyl derived residue ratio nor the ratio of oxidized to non-oxidized lignin residues. Moreover, the carbohydrate content and accessibility was not affected in compost. Notably, the capacity of compost extract to consume the MnP co-factor H

Keywords: Agaricus bisporus; Compost; Fungus; Hydrogen peroxide; Lignin; Manganese peroxidase

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