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Cao GP, Yang RY, Zhuang YF, et al. Simple and sensitive determination of trace nitrite in water by zero-crossing first-derivative synchronous fluorescence spectrometry using 6-amino-1,3- naphthalenedisulfonic acid as a new fluorescent probe. Anal Bioanal Chem. 2017;409(19):4637-4646doi: 10.1007/s00216-017-0409-4.
Cao, G. P., Yang, R. Y., Zhuang, Y. F., Zuo, D., & Wang, Y. H. (2017). Simple and sensitive determination of trace nitrite in water by zero-crossing first-derivative synchronous fluorescence spectrometry using 6-amino-1,3- naphthalenedisulfonic acid as a new fluorescent probe. Analytical and bioanalytical chemistry, 409(19), 4637-4646. https://doi.org/10.1007/s00216-017-0409-4
Cao, Gui-Ping, et al. "Simple and sensitive determination of trace nitrite in water by zero-crossing first-derivative synchronous fluorescence spectrometry using 6-amino-1,3- naphthalenedisulfonic acid as a new fluorescent probe." Analytical and bioanalytical chemistry vol. 409,19 (2017): 4637-4646. doi: https://doi.org/10.1007/s00216-017-0409-4
Cao GP, Yang RY, Zhuang YF, Zuo D, Wang YH. Simple and sensitive determination of trace nitrite in water by zero-crossing first-derivative synchronous fluorescence spectrometry using 6-amino-1,3- naphthalenedisulfonic acid as a new fluorescent probe. Anal Bioanal Chem. 2017 Jul;409(19):4637-4646. doi: 10.1007/s00216-017-0409-4. Epub 2017 Jun 02. PMID: 28573318.
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Anal Bioanal Chem. 2017 Jul;409(19):4637-4646. doi: 10.1007/s00216-017-0409-4. Epub 2017 Jun 02.

Simple and sensitive determination of trace nitrite in water by zero-crossing first-derivative synchronous fluorescence spectrometry using 6-amino-1,3- naphthalenedisulfonic acid as a new fluorescent probe.

Analytical and bioanalytical chemistry

Gui-Ping Cao, Run-Ye Yang, Ya-Feng Zhuang, Di Zuo, Yan-Hua Wang

Affiliations

  1. Department of Chemical Engineering, Changzhou Institute of Technology, Changzhou, Jiangsu, 213032, China. [email protected].
  2. Department of Chemical Engineering, Changzhou Institute of Technology, Changzhou, Jiangsu, 213032, China.
  3. School of Geography Science, Nanjing Normal University, Nanjing, Jiangsu, 210023, China.

PMID: 28573318 DOI: 10.1007/s00216-017-0409-4

Abstract

A new fluorescent probe, 6-amino-1,3-naphthalenedisulfonic acid (ANDSA), has been developed for the determination of trace nitrite in different waters. This probe is based on the selective reaction of nitrite with ANDSA in hydrochloric acid solution to form the corresponding diazonium acid in sodium hydroxide solution at room temperature. The diazonium acid produced has high fluorescence intensity at 488 nm (excitation at 367 nm), whereas ANDSA has high fluorescence intensity at 465 nm (excitation at 354 nm). The synchronous fluorescence (Δλ = 121 nm) spectrum and the first-derivative synchronous fluorescence spectrum of diazonium acid greatly overlapped with those of ANDSA. The zero-crossing method was used to measure the first-derivative value (dF/dλ) of the first-derivative spectra, in which physical separation of excess ANDSA was unnecessary. The zero-crossing point was located at 351.2 nm for ANDSA, at which dF/dλ of diazonium acid was proportional to the nitrite concentration. Therefore, dF/dλ at 351.2 nm was selected as the analytical signal. Under the optimized experimental conditions, the signal intensity was linear over a nitrite concentration range of 0.006-0.075 mg L

Keywords: 6-Amino-1,3-naphthalenedisulfonic acid; First-derivative synchronous fluorescence spectrometry; Fluorescent probe; Nitrite; Zero-crossing method

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