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Huang J, Wang D, Chen J, et al. Osteogenic differentiation of bone marrow mesenchymal stem cells by magnetic nanoparticle composite scaffolds under a pulsed electromagnetic field. Saudi Pharm J. 2017;25(4):575-579doi: 10.1016/j.jsps.2017.04.026.
Huang, J., Wang, D., Chen, J., Liu, W., Duan, L., You, W., Zhu, W., Xiong, J., & Wang, D. (2017). Osteogenic differentiation of bone marrow mesenchymal stem cells by magnetic nanoparticle composite scaffolds under a pulsed electromagnetic field. Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 25(4), 575-579. https://doi.org/10.1016/j.jsps.2017.04.026
Huang, Jianghong, et al. "Osteogenic differentiation of bone marrow mesenchymal stem cells by magnetic nanoparticle composite scaffolds under a pulsed electromagnetic field." Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society vol. 25,4 (2017): 575-579. doi: https://doi.org/10.1016/j.jsps.2017.04.026
Huang J, Wang D, Chen J, Liu W, Duan L, You W, Zhu W, Xiong J, Wang D. Osteogenic differentiation of bone marrow mesenchymal stem cells by magnetic nanoparticle composite scaffolds under a pulsed electromagnetic field. Saudi Pharm J. 2017 May;25(4):575-579. doi: 10.1016/j.jsps.2017.04.026. Epub 2017 May 08. PMID: 28579894; PMCID: PMC5447436.
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Saudi Pharm J. 2017 May;25(4):575-579. doi: 10.1016/j.jsps.2017.04.026. Epub 2017 May 08.

Osteogenic differentiation of bone marrow mesenchymal stem cells by magnetic nanoparticle composite scaffolds under a pulsed electromagnetic field.

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society

Jianghong Huang, Daming Wang, Jielin Chen, Wei Liu, Li Duan, Wei You, Weimin Zhu, Jianyi Xiong, Daping Wang

Affiliations

  1. Shenzhen National Key Department of Orthopedics, Shenzhen Second People's Hospital, Shenzhen 518035, China.
  2. Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China.
  3. Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China.
  4. Software Town of Shenzhen Universiade, Shenzhen Longer3d Technology, Shenzhen 518116, China.

PMID: 28579894 PMCID: PMC5447436 DOI: 10.1016/j.jsps.2017.04.026

Abstract

This study was conducted to investigate the effect of magnetic nanoparticle composite scaffold under a pulsed electromagnetic field on bone marrow mesenchymal stem cells (BMSCs), which was achieved by examining the biological behaviors of cell adhesion, proliferation and differentiation on the surface of the scaffolds. This may provide some experimental evidence for the use of magnetic nanoparticles in medical application. The magnetic nanoparticle composite scaffolds were evaluated and characterized by the following indexes: the cell proliferation was detected by the CCK-8 method, the alkaline phosphatase (ALP) activity was examined by a detection kit, and the expression of type I collagen and osteocalcin gene were evaluated by RT-PCR. The CCK-8 test showed that there was no significant difference in Group A (BMSCs-seeded magnetic scaffolds under the electromagnetic field), B (BMSCs-seeded magnetic scaffolds) and C (BMSCs cultured alone) (P > 0.05). The value for the ALP activity in Group A was higher than the other two groups. In addition, the RT-PCR results showed that the expression of type I collagen gene in Group A was enhanced (P < 0.05), suggesting that the magnetic nanoparticles combined with the pulsed electromagnetic field had a positive effect on the osteogenic differentiation of BMSCs. However, the expression of osteocalcin was not significantly different in three groups (P > 0.05). To conclude, magnetic nanoparticles may induce the osteogenic differentiation with the action of the pulsed electromagnetic field.

Keywords: Bone marrow mesenchymal stem cells; Magnetic nanoparticles; Osteogenic differentiation; Pulsed electromagnetic field

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