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Li X, Mohammadi AS, Ewing AG. Single cell amperometry reveals curcuminoids modulate the release of neurotransmitters during exocytosis from PC12 cells. J Electroanal Chem (Lausanne). 2016;781:30-35doi: 10.1016/j.jelechem.2016.10.025.
Li, X., Mohammadi, A. S., & Ewing, A. G. (2016). Single cell amperometry reveals curcuminoids modulate the release of neurotransmitters during exocytosis from PC12 cells. Journal of electroanalytical chemistry (Lausanne, Switzerland), 78130-35. https://doi.org/10.1016/j.jelechem.2016.10.025
Li, Xianchan, et al. "Single cell amperometry reveals curcuminoids modulate the release of neurotransmitters during exocytosis from PC12 cells." Journal of electroanalytical chemistry (Lausanne, Switzerland) vol. 781 (2016): 30-35. doi: https://doi.org/10.1016/j.jelechem.2016.10.025
Li X, Mohammadi AS, Ewing AG. Single cell amperometry reveals curcuminoids modulate the release of neurotransmitters during exocytosis from PC12 cells. J Electroanal Chem (Lausanne). 2016 Nov 15;781:30-35. doi: 10.1016/j.jelechem.2016.10.025. Epub 2016 Oct 13. PMID: 28579928; PMCID: PMC5450664.
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J Electroanal Chem (Lausanne). 2016 Nov 15;781:30-35. doi: 10.1016/j.jelechem.2016.10.025. Epub 2016 Oct 13.

Single cell amperometry reveals curcuminoids modulate the release of neurotransmitters during exocytosis from PC12 cells.

Journal of electroanalytical chemistry (Lausanne, Switzerland)

Xianchan Li, Amir Saeid Mohammadi, Andrew G Ewing

Affiliations

  1. Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, 41296 Gothenburg, Sweden.
  2. Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemivägen 10, 41296 Gothenburg, Sweden.

PMID: 28579928 PMCID: PMC5450664 DOI: 10.1016/j.jelechem.2016.10.025

Abstract

We used single cell amperometry to examine whether curcumin and bisdemethoxycurcumin (BDMC), substances that are suggested to affect learning and memory, can modulate monoamine release from PC12 cells. Our results indicate both curcumin and BDMC need long-term treatment (72 h in this study) to influence exocytosis effectively. By analyzing the parameters calculated from single exocytosis events, it can be concluded that curcumin and BDMC affect exocytosis through different mechanisms. Curcumin accelerates the event dynamics with no significant change of the monoamine amount released from single exocytotic events, whereas BDMC attenuates the amount from single exocytotic event with no significant change of the event dynamics. This comparison of the effect of curcumin and BDMC on exocytosis at the single cell level brings insight into their different mechanisms, which might lead to different biological actions. The effect of curcumin and BDMC on the opening and closing of the exocytotic fusion pore were also investigated. These results might be helpful for understanding the improvement of learning and memory and the anti-depression properties of curcuminoids.

Keywords: Bisdemethoxycurcumin; Curcumin; Exocytosis; Model cells; PC12 cells; Single cell amperometry

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