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Venkateswarulu TC, Prabhakar KV, Kumar RB. Optimization of nutritional components of medium by response surface methodology for enhanced production of lactase. 3 Biotech. 2017;7(3):202doi: 10.1007/s13205-017-0805-7.
Venkateswarulu, T. C., Prabhakar, K. V., & Kumar, R. B. (2017). Optimization of nutritional components of medium by response surface methodology for enhanced production of lactase. 3 Biotech, 7(3), 202. https://doi.org/10.1007/s13205-017-0805-7
Venkateswarulu, T C, et al. "Optimization of nutritional components of medium by response surface methodology for enhanced production of lactase." 3 Biotech vol. 7,3 (2017): 202. doi: https://doi.org/10.1007/s13205-017-0805-7
Venkateswarulu TC, Prabhakar KV, Kumar RB. Optimization of nutritional components of medium by response surface methodology for enhanced production of lactase. 3 Biotech. 2017 Jul;7(3):202. doi: 10.1007/s13205-017-0805-7. Epub 2017 Jun 30. PMID: 28667642; PMCID: PMC5493568.
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3 Biotech. 2017 Jul;7(3):202. doi: 10.1007/s13205-017-0805-7. Epub 2017 Jun 30.

Optimization of nutritional components of medium by response surface methodology for enhanced production of lactase.

3 Biotech

T C Venkateswarulu, K Vidya Prabhakar, R Bharath Kumar

Affiliations

  1. Department of Biotechnology, Vignan's Foundation for Science, Technology and Research University, Vadlamudi, Andhra Pradesh, 522213, India. [email protected].
  2. Department of Biotechnology, Vikrama Simhapuri University, Nellore, Andhra Pradesh, 524003, India.
  3. Department of Biotechnology, Vignan's Foundation for Science, Technology and Research University, Vadlamudi, Andhra Pradesh, 522213, India.

PMID: 28667642 PMCID: PMC5493568 DOI: 10.1007/s13205-017-0805-7

Abstract

Lactase has excellent applications in dairy industry and commercially this enzyme is produced from bacterial sources but not in high yields. In this work, the production of lactase was improved by designing of nutrient components in fermentation medium by one factor at a time. Lactose and yeast extract were selected as preferable carbon and nitrogen sources for lactase production with tryptophan and MgSO

Keywords: B. subtilis strain VUVD001; Lactase; Response surface methodology; Shake flask culture; Yeast extract

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