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Escobar-Chaparro RA, Guillén G, Espejo-Galicia LU, et al. qPCR and HRM-based diagnosis of SNPs on growth differentiation factor 9 (GDF9), a gene associated with sheep (Ovis aries) prolificacy. 3 Biotech. 2017;7(3):204doi: 10.1007/s13205-017-0837-z.
Escobar-Chaparro, R. A., Guillén, G., Espejo-Galicia, L. U., Meza-Villalvazo, V. M., Peña-Castro, J. M., & Abad-Zavaleta, J. (2017). qPCR and HRM-based diagnosis of SNPs on growth differentiation factor 9 (GDF9), a gene associated with sheep (Ovis aries) prolificacy. 3 Biotech, 7(3), 204. https://doi.org/10.1007/s13205-017-0837-z
Escobar-Chaparro, Raquel Anahí, et al. "qPCR and HRM-based diagnosis of SNPs on growth differentiation factor 9 (GDF9), a gene associated with sheep (Ovis aries) prolificacy." 3 Biotech vol. 7,3 (2017): 204. doi: https://doi.org/10.1007/s13205-017-0837-z
Escobar-Chaparro RA, Guillén G, Espejo-Galicia LU, Meza-Villalvazo VM, Peña-Castro JM, Abad-Zavaleta J. qPCR and HRM-based diagnosis of SNPs on growth differentiation factor 9 (GDF9), a gene associated with sheep (Ovis aries) prolificacy. 3 Biotech. 2017 Jul;7(3):204. doi: 10.1007/s13205-017-0837-z. Epub 2017 Jun 30. PMID: 28667646; PMCID: PMC5493575.
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3 Biotech. 2017 Jul;7(3):204. doi: 10.1007/s13205-017-0837-z. Epub 2017 Jun 30.

qPCR and HRM-based diagnosis of SNPs on growth differentiation factor 9 (GDF9), a gene associated with sheep (Ovis aries) prolificacy.

3 Biotech

Raquel Anahí Escobar-Chaparro, Gabriel Guillén, Luis Uribe Espejo-Galicia, Víctor Manuel Meza-Villalvazo, Julián Mario Peña-Castro, José Abad-Zavaleta

Affiliations

  1. División de Estudios de Posgrado, Universidad del Papaloapan, Tuxtepec, Oaxaca, Mexico.
  2. Instituto de Biotecnología, Universidad del Papaloapan, Circuito Central No. 200, Parque Industrial, C.P. 68301, Tuxtepec, Oaxaca, Mexico.
  3. Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
  4. Instituto de Biotecnología, Universidad del Papaloapan, Circuito Central No. 200, Parque Industrial, C.P. 68301, Tuxtepec, Oaxaca, Mexico. [email protected].

PMID: 28667646 PMCID: PMC5493575 DOI: 10.1007/s13205-017-0837-z

Abstract

Prolificacy is a desirable trait for genetic improvement of sheep flocks, since it holds the potential to improve productivity. Animals carrying single-nucleotide polymorphisms (SNPs) in genes associated with this trait can be identified and employed to increase prolificacy in flocks. In this study, we report a diagnostic method based on quantitative PCR and high-resolution melting curves to detect different SNPs in the prolificacy-associated gene growth differentiation factor 9 (GDF9). The diagnostic method was validated using artificial sequences representing known SNPs in GDF9, then applied to a real flock comprising four breeds and admixed animals (n = 306). Five different SNPs were identified in this flock, as was a low or null frequency of occurrence of SNPs positively associated with prolificacy. This indicates a need to implement a breeding strategy for recovering or reintroducing such SNPs. Our method provides a genotyping strategy for identifying individuals with SNPs of interest for prolificacy, which will help producers plan a breeding strategy for this trait. This method can be adapted and expanded for the diagnosis of other traits of interest.

Keywords: Animal breeding; Fertility; Genomic DNA; Genotyping

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