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Fujino Y, Minamizaki T, Yoshioka H, et al. Imaging and mapping of mouse bone using MALDI-imaging mass spectrometry. Bone Rep. 2016;5:280-285doi: 10.1016/j.bonr.2016.09.004.
Fujino, Y., Minamizaki, T., Yoshioka, H., Okada, M., & Yoshiko, Y. (2016). Imaging and mapping of mouse bone using MALDI-imaging mass spectrometry. Bone reports, 5280-285. https://doi.org/10.1016/j.bonr.2016.09.004
Fujino, Yoko, et al. "Imaging and mapping of mouse bone using MALDI-imaging mass spectrometry." Bone reports vol. 5 (2016): 280-285. doi: https://doi.org/10.1016/j.bonr.2016.09.004
Fujino Y, Minamizaki T, Yoshioka H, Okada M, Yoshiko Y. Imaging and mapping of mouse bone using MALDI-imaging mass spectrometry. Bone Rep. 2016 Sep 29;5:280-285. doi: 10.1016/j.bonr.2016.09.004. eCollection 2016 Dec. PMID: 28580397; PMCID: PMC5440778.
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Bone Rep. 2016 Sep 29;5:280-285. doi: 10.1016/j.bonr.2016.09.004. eCollection 2016 Dec.

Imaging and mapping of mouse bone using MALDI-imaging mass spectrometry.

Bone reports

Yoko Fujino, Tomoko Minamizaki, Hirotaka Yoshioka, Mitsugi Okada, Yuji Yoshiko

Affiliations

  1. Department of Special Care Dentistry, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
  2. Department of Calcified Tissue Biology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
  3. Special Care Dentistry, Hiroshima University Hospital, Hiroshima, Japan.

PMID: 28580397 PMCID: PMC5440778 DOI: 10.1016/j.bonr.2016.09.004

Abstract

Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is an advanced method used globally to analyze the distribution of biomolecules on tissue cryosections without any probes. In bones, however, hydroxyapatite crystals make it difficult to determine the distribution of biomolecules using MALDI-IMS. Additionally, there is limited information regarding the use of this method to analyze bone tissues. To determine whether MALDI-IMS analysis of bone tissues can facilitate comprehensive mapping of biomolecules in mouse bone, we first dissected femurs and tibiae from 8-week-old male mice and characterized the quality of multiple fixation and decalcification methods for preparation of the samples. Cryosections were mounted on indium tin oxide-coated glass slides, dried, and then a matrix solution was sprayed on the tissue surface. Images were acquired using an iMScope at a mass-to-charge range of 100-1000. Hematoxylin-eosin, Alcian blue, Azan, and periodic acid-Schiff staining of adjacent sections was used to evaluate histological and histochemical features. Among the various fixation and decalcification conditions, sections from trichloroacetic acid-treated samples were most suitable to examine both histology and comprehensive MS images. However, histotypic MS signals were detected in all sections. In addition to the MS images, phosphocholine was identified as a candidate metabolite. These results indicate successful detection of biomolecules in bone using MALDI-IMS. Although analytical procedures and compositional adjustment regarding the performance of the device still require further development, IMS appears to be a powerful tool to determine the distribution of biomolecules in bone tissues.

Keywords: Bone; Decalcification; Fixation; Matrix-assisted laser desorption/ionization-imaging mass spectrometry; Tissue cryosection

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