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Mirhoseini M, Talebpour Amiri F, Karimpour Malekshah AA, et al. Protective effects of melatonin on testis histology following acute torsion-detorsion in rats. Int J Reprod Biomed. 2017;15(3):141-146
Mirhoseini, M., Talebpour Amiri, F., Karimpour Malekshah, A. A., Rezanejad Gatabi, Z., & Ghaffari, E. (2017). Protective effects of melatonin on testis histology following acute torsion-detorsion in rats. International journal of reproductive biomedicine, 15(3), 141-146.
Mirhoseini, Mehri, et al. "Protective effects of melatonin on testis histology following acute torsion-detorsion in rats." International journal of reproductive biomedicine vol. 15,3 (2017): 141-146.
Mirhoseini M, Talebpour Amiri F, Karimpour Malekshah AA, Rezanejad Gatabi Z, Ghaffari E. Protective effects of melatonin on testis histology following acute torsion-detorsion in rats. Int J Reprod Biomed. 2017 Mar;15(3):141-146. PMID: 28580446; PMCID: PMC5447830.
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Int J Reprod Biomed. 2017 Mar;15(3):141-146.

Protective effects of melatonin on testis histology following acute torsion-detorsion in rats.

International journal of reproductive biomedicine

Mehri Mirhoseini, Fereshteh Talebpour Amiri, Abbas Ali Karimpour Malekshah, Zahra Rezanejad Gatabi, Elmira Ghaffari

Affiliations

  1. Amol Faculty of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran.
  2. Department of Anatomical Sciences, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  3. Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

PMID: 28580446 PMCID: PMC5447830

Abstract

BACKGROUND: Testicular damage due to spermatic cord torsion may lead to infertility. It is probably because of changes in oxidative stress factors such as malondialdehyde.

OBJECTIVE: To investigate the protective effect of melatonin (MLT), as an antioxidant, on testicular damage induced by acute unilateral spermatic cord torsion and detorsion (T/D) in rats.

MATERIALS AND METHODS: In this experimental study, 48 adult male Wistar rats were randomly divided into three groups (8 rats/group): sham group underwent right scrotal surgery only., the T/D group underwent right testicular torsion (for 1 hr) and detorsion, and the melatonin group underwent right testicular torsion, received 25 µg/kg melatonin intraperitoneally immediately after surgery of T/D. Then the histological parameters and malondialdehyde (MDA) changes were evaluated.

RESULTS: Torsion and detorsion decreased the diameter of the tubules significantly compared to controls (p=0.003). Melatonin could increase the diameter, but it was not significant (p=0.26). The heights of the epithelium were constant in sham, T/D, and melatonin groups without any significant difference between groups (p=0.98). Based on Johnsen's score, spermatogenesis was normal in the sham group. The torsion significantly injured all lineage cells (p<0.001). There was no any spermatid or sperm in the seminiferous tubules. Melatonin improved the spermatogenesis significantly (p=0.02), but could not improve MDA level significantly (p=0.99).

CONCLUSION: Severe degenerative changes of testis were induced by acute unilateral spermatic cord torsion and detorsion in rats, but it had no effect on MDA level.

Keywords: MDA; Melatonin; Rats; Spermatic cord torsion; Testis

Conflict of interest statement

The authors disclose no financial or other conflicts of interest.

References

  1. Folia Morphol (Warsz). 2011 May;70(2):95-102 - PubMed
  2. J Pediatr Surg. 2007 Apr;42(4):699-703 - PubMed
  3. Sao Paulo Med J. 2015 May-Jun;133(3):187-90 - PubMed
  4. Cancer Cell Int. 2015 Dec 18;15:121 - PubMed
  5. Exp Clin Endocrinol Diabetes. 1996;104(1):10-6 - PubMed
  6. Life Sci. 1994;55(15):PL271-6 - PubMed
  7. Can J Surg. 2010 Oct;53(5):313-8 - PubMed
  8. J Pediatr Surg. 2009 Sep;44(9):1754-8 - PubMed
  9. J Pediatr Surg. 1997 Jan;32(1):84-7 - PubMed
  10. Urology. 2002 Sep;60(3):548-51 - PubMed
  11. Biol Reprod. 1993 Aug;49(2):401-7 - PubMed
  12. J Urol. 1990 Aug;144(2 Pt 1):366-9 - PubMed
  13. J Assist Reprod Genet. 2012 May;29(5):457-61 - PubMed
  14. J Pineal Res. 1994 May;16(4):198-201 - PubMed
  15. J Pediatr Urol. 2016 Dec;12(6):410.e1-410.e7 - PubMed
  16. Eur Rev Med Pharmacol Sci. 2012 May;16(5):582-8 - PubMed
  17. West Afr J Med. 2007 Oct-Dec;26(4):312-5 - PubMed
  18. J Pediatr Surg. 2017 Apr;52(4):582-586 - PubMed
  19. Fertil Steril. 2008 May;89(5 Suppl):1468-73 - PubMed
  20. J Pediatr Surg. 2008 Oct;43(10):1873-8 - PubMed
  21. J Pediatr Surg. 2004 Aug;39(8):1238-41 - PubMed
  22. Asian J Androl. 2014 Mar-Apr;16(2):314-8 - PubMed
  23. Br J Urol. 1986 Dec;58(6):698-704 - PubMed
  24. Exp Mol Pathol. 2010 Dec;89(3):314-20 - PubMed
  25. Iran Red Crescent Med J. 2014 Feb;16(2):e14463 - PubMed
  26. Int J Exp Pathol. 2015 Oct;96(5):294-300 - PubMed
  27. Pharmacol Res. 2001 Aug;44(2):89-93 - PubMed
  28. Lancet. 1988 Apr 30;1(8592):955-60 - PubMed

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