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Chung J, Sharif F, Jung D, et al. Micro-drive and headgear for chronic implant and recovery of optoelectronic probes. Sci Rep. 2017;7(1):2773doi: 10.1038/s41598-017-03340-5.
Chung, J., Sharif, F., Jung, D., Kim, S., & Royer, S. (2017). Micro-drive and headgear for chronic implant and recovery of optoelectronic probes. Scientific reports, 7(1), 2773. https://doi.org/10.1038/s41598-017-03340-5
Chung, Jinho, et al. "Micro-drive and headgear for chronic implant and recovery of optoelectronic probes." Scientific reports vol. 7,1 (2017): 2773. doi: https://doi.org/10.1038/s41598-017-03340-5
Chung J, Sharif F, Jung D, Kim S, Royer S. Micro-drive and headgear for chronic implant and recovery of optoelectronic probes. Sci Rep. 2017 Jun 05;7(1):2773. doi: 10.1038/s41598-017-03340-5. PMID: 28584246; PMCID: PMC5459843.
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Sci Rep. 2017 Jun 05;7(1):2773. doi: 10.1038/s41598-017-03340-5.

Micro-drive and headgear for chronic implant and recovery of optoelectronic probes.

Scientific reports

Jinho Chung, Farnaz Sharif, Dajung Jung, Soyoun Kim, Sebastien Royer

Affiliations

  1. Center for Functional Connectomics, Korea Institute of Science and Technology, Seoul, 136-791, Republic of Korea.
  2. University of Science & Technology, Daejeon, Republic of Korea.
  3. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea.
  4. Center for Functional Connectomics, Korea Institute of Science and Technology, Seoul, 136-791, Republic of Korea. [email protected].
  5. University of Science & Technology, Daejeon, Republic of Korea. [email protected].

PMID: 28584246 PMCID: PMC5459843 DOI: 10.1038/s41598-017-03340-5

Abstract

Silicon probes are multisite electrodes used for the electrophysiological recording of large neuronal ensembles. Optoelectronic probes (OEPs) are recent upgrades that allow, in parallel, the delivery of local optical stimuli. The procedures to use these delicate electrodes for chronic experiments in mice are still underdeveloped and typically assume one-time uses. Here, we developed a micro-drive, a support for OEPs optical fibers, and a hat enclosure, which fabrications consist in fitting and fastening together plastic parts made with 3D printers. Excluding two parts, all components and electrodes are relatively simple to recover after the experiments, via the loosening of screws. To prevent the plugging of OEPs laser sources from altering the stability of recordings, the OEPs fibers can be transiently anchored to the hat via the tightening of screws. We test the stability of recordings in the mouse hippocampus under three different conditions: acute head-fixed, chronic head-fixed, and chronic freely moving. Drift in spike waveforms is significantly smaller in chronic compared to acute conditions, with the plugging/unplugging of head-stage and fiber connectors not affecting much the recording stability. Overall, these tools generate stable recordings of place cell in chronic conditions, and make the recovery and reuse of electrode packages relatively simple.

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