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Chen CM, Lai CH, Wu HJ, et al. Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples. Biomedicine (Taipei). 2017;7(2):9doi: 10.1051/bmdcn/2017070202.
Chen, C. M., Lai, C. H., Wu, H. J., & Wu, L. T. (2017). Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples. BioMedicine, 7(2), 9. https://doi.org/10.1051/bmdcn/2017070202
Chen, Chih-Ming, et al. "Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples." BioMedicine vol. 7,2 (2017): 9. doi: https://doi.org/10.1051/bmdcn/2017070202
Chen CM, Lai CH, Wu HJ, Wu LT. Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples. Biomedicine (Taipei). 2017 Jun;7(2):9. doi: 10.1051/bmdcn/2017070202. Epub 2017 Jun 14. PMID: 28612707; PMCID: PMC5479437.
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Biomedicine (Taipei). 2017 Jun;7(2):9. doi: 10.1051/bmdcn/2017070202. Epub 2017 Jun 14.

Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples.

BioMedicine

Chih-Ming Chen, Chih-Ho Lai, Hwa-Jene Wu, Lii-Tzu Wu

Affiliations

  1. Division of Infectious Disease, Department of Internal Medicine, Tungs' Taichung MetroHarbor Hospital, Taichung 433, Taiwan - Department of Health Food, Chung Chou University of Science and Technology, Changhua 510, Taiwan.
  2. Graduate Institute of Biomedical Sciences, Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan - Molecular Infectious Disease Research Center, Department of Pediatrics, Chang Gung Children's Hospital and Chang Gung Memorial Hospital, Taoyuan 333, Taiwan - Graduate Institute of Basic Medical Science, School of Medicine, China Medical University, Taichung 404, Taiwan - Department of Medical Research and Department of Laboratory Medicine, China Medical University Hospital, Taichung 404, Taiwan - Department of Nursing, Asia University, Taichung 413, Taiwan.
  3. Department of Clinical Laboratory, Jen-Ai Hospital, Taichung 412, Taiwan.
  4. Graduate Institute of Basic Medical Science, School of Medicine, China Medical University, Taichung 404, Taiwan.

PMID: 28612707 PMCID: PMC5479437 DOI: 10.1051/bmdcn/2017070202

Abstract

BACKGROUND: Proteus mirabilis is an opportunistic pathogen, commonly associated with complicated urinary tract infections (UTIs). UTIs caused by multidrug-resistant Proteus mirabilis have increased worldwide. Multidrug-resistance of Gram-negative enteric bacteria is usually associated with class 1 integrons.

PURPOSES: To investigate the prevalence and characterize gene cassettes of class 1 integrons in multidrug-resistant P. mirabilis Methods: From 2006 to 2008, 314 P. mirabilis isolates from urine were collected from a regional teaching hospital. Antimicrobial resistance of the isolates was determined by disk diffusion methods. The phenotypic confirmatory test of extended-spectrum β-lactamase (ESBL) production was performed as described in the Clinical and Laboratory Standards Institute (CLSI) guideline. The genetic organization of the class 1 integron cassettes was investigated by PCR, cloning, and sequencing of the regions surrounding these genes.

RESULTS: Seventy-nine (25%, 79/314) P. mirabilis isolates were ESBL-producing and most ESBL-producing P. mirabilis were positive for bla

CONCLUSION: This study investigated the prevalence and characterized gene cassettes of class 1 integrons in MDR P. mirabilis isolates from urine samples. The frequency of gene cassettes in P. mirabilis were partially by clonal spread of the carriers and the results could provide information for effective antimicrobial therapy and infection control.

© Author(s) 2017. This article is published with open access by China Medical University.

References

  1. Antimicrob Agents Chemother. 2005 Nov;49(11):4771-4 - PubMed
  2. Int J Antimicrob Agents. 2015 Jan;45(1):84-5 - PubMed
  3. Int J Antimicrob Agents. 2009 Mar;33(3):216-22 - PubMed
  4. Clin Microbiol Infect. 2004 Apr;10(4):272-88 - PubMed
  5. Microbiology. 2015 Jul;161(7):1313-37 - PubMed
  6. Antimicrob Agents Chemother. 2000 Aug;44(8):2023-7 - PubMed
  7. Antimicrob Agents Chemother. 2013 Jul;57(7):2981-8 - PubMed
  8. Int J Antimicrob Agents. 2001 Feb;17(2):131-5 - PubMed
  9. Clin Microbiol Infect. 2013 Feb;19(2):E51-8 - PubMed
  10. J Clin Microbiol. 2000 Feb;38(2):542-6 - PubMed
  11. Nucleic Acids Res. 1991 Dec 25;19(24):6823-31 - PubMed
  12. Clin Microbiol Infect. 2008 Jan;14 Suppl 1:75-81 - PubMed
  13. Diagn Microbiol Infect Dis. 2006 Feb;54(2):89-94 - PubMed
  14. J Clin Microbiol. 2008 Sep;46(9):3080-3 - PubMed
  15. J Antimicrob Chemother. 2007 Jul;60(1):136-9 - PubMed
  16. FEMS Microbiol Rev. 2009 Jul;33(4):757-84 - PubMed
  17. Mol Microbiol. 1995 Feb;15(4):593-600 - PubMed
  18. Int J Antimicrob Agents. 2009 Apr;33(4):328-33 - PubMed
  19. BMC Infect Dis. 2014 Sep 05;14:486 - PubMed
  20. Clin Microbiol Infect. 2009 Jan;15 Suppl 1:5-10 - PubMed
  21. Kaohsiung J Med Sci. 2007 Jun;23(6):273-80 - PubMed
  22. J Antimicrob Chemother. 2005 May;55(5):639-44 - PubMed
  23. Int J Antimicrob Agents. 2015 May;45(5):485-90 - PubMed
  24. Nat Rev Microbiol. 2006 Aug;4(8):608-20 - PubMed

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