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Mirjanic-Azaric B, Avram S, Stojakovic-Jelisavac T, et al. Direct Estimation of Reference Intervals for Thyroid Parameters in the Republic of Srpska. J Med Biochem. 2017;36(2):137-144doi: 10.1515/jomb-2017-0008.
Mirjanic-Azaric, B., Avram, S., Stojakovic-Jelisavac, T., Stojanovic, D., Petkovic, M., Bogavac-Stanojevic, N., Ignjatovic, S., & Stojanov, M. (2017). Direct Estimation of Reference Intervals for Thyroid Parameters in the Republic of Srpska. Journal of medical biochemistry, 36(2), 137-144. https://doi.org/10.1515/jomb-2017-0008
Mirjanic-Azaric, Bosa, et al. "Direct Estimation of Reference Intervals for Thyroid Parameters in the Republic of Srpska." Journal of medical biochemistry vol. 36,2 (2017): 137-144. doi: https://doi.org/10.1515/jomb-2017-0008
Mirjanic-Azaric B, Avram S, Stojakovic-Jelisavac T, Stojanovic D, Petkovic M, Bogavac-Stanojevic N, Ignjatovic S, Stojanov M. Direct Estimation of Reference Intervals for Thyroid Parameters in the Republic of Srpska. J Med Biochem. 2017 Apr 22;36(2):137-144. doi: 10.1515/jomb-2017-0008. eCollection 2017 Apr. PMID: 28680357; PMCID: PMC5471646.
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J Med Biochem. 2017 Apr 22;36(2):137-144. doi: 10.1515/jomb-2017-0008. eCollection 2017 Apr.

Direct Estimation of Reference Intervals for Thyroid Parameters in the Republic of Srpska.

Journal of medical biochemistry

Bosa Mirjanic-Azaric, Sanja Avram, Tanja Stojakovic-Jelisavac, Darja Stojanovic, Mira Petkovic, Natasa Bogavac-Stanojevic, Svetlana Ignjatovic, Marina Stojanov

Affiliations

  1. University Clinical Centre of the Republic of SrpskaBanja Luka, Bosnia and Herzegovina, Serbia.
  2. Health Centre Laktasi, the Republic of Srpska, Bosnia and HerzegovinaSerbia.
  3. Institute for Sport and Occupational Medicine Banja Luka, Bosnia and HerzegovinaSerbia.
  4. Institute of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Serbia.
  5. Center for Medical Biochemistry, Clinical Center of Serbia, Belgrade, Serbia.

PMID: 28680357 PMCID: PMC5471646 DOI: 10.1515/jomb-2017-0008

Abstract

BACKGROUND: The aim of this study was to determine the reference values for thyrotropin (TSH), thyroid hormones (total and free thyroxine, T4 and fT4; total and free triiodothyronine, T3 and fT3), thyroglobulin (Tg) and thyroid antibodies (thyroid peroxidase, TPOAb and thyroglobulin antibody, TgAb) in the population of the Republic of Srpska.

METHODS: A total of 250 euthyroid subjects were enrolled in this study. A direct method for choosing reference subjects was used to establish reference intervals. The hormones and thyroid antibodies were measured by an electrochemiluminescence immunoassay method (ECLIA, Roche Diagnostics, Mannheim, Germany). We calculated the reference intervals by MedCalc, version 12.1.4.0 (MedCalc software, Belgium) as recommended by the IFCC (CLSI C28-A3).

RESULTS: Using guidelines recommended by the National Academy of Clinical Biochemistry (NACB) and based on standard statistical approaches, the reference intervals derived for TSH, fT4, T4, fT3, T3 were 0.75-5.32 mIU/L, 12.29-20.03 pmol/L, 73.49-126,30 nmol/L, 4.11-6.32 pmol/L, 1.15-2.32 nmol/L and for Tg, TPOAb, TgAb were 3.63-26.00 μg/L, <18.02 mIU/L, < 98.00 mIU/L, respectively. We found a significant difference (p<0.05) in TSH and fT3 values between different age groups as well as in T4, fT4 and fT3 values between ge nder groups.

CONCLUSIONS: The established reference values for the population of the Republic of Srpska were significantly different from the values recommended by the manufacturer of reagents (Roche Diagnostics). Our results showed that a laboratory needs to establish its own reference values in order to set up a proper diagnosis, as well as to treat patients successfully.

Keywords: TSH; reference values; thyroid hormones

Conflict of interest statement

Conflict of interest statement: The authors stated that they have no conflicts of interest regarding the publication of this article.

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