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Grèze V, Kanold J, Chambon F, et al. RT-qPCR for . Oncol Lett. 2017;14(1):860-866doi: 10.3892/ol.2017.6238.
Grèze, V., Kanold, J., Chambon, F., Halle, P., Gremeau, A. S., Rives, N., Rouel, N., Pereira, B., Tchirkov, A., & Brugnon, F. (2017). RT-qPCR for . Oncology letters, 14(1), 860-866. https://doi.org/10.3892/ol.2017.6238
Grèze, Victoria, et al. "RT-qPCR for ." Oncology letters vol. 14,1 (2017): 860-866. doi: https://doi.org/10.3892/ol.2017.6238
Grèze V, Kanold J, Chambon F, Halle P, Gremeau AS, Rives N, Rouel N, Pereira B, Tchirkov A, Brugnon F. RT-qPCR for . Oncol Lett. 2017 Jul;14(1):860-866. doi: 10.3892/ol.2017.6238. Epub 2017 May 24. PMID: 28693243; PMCID: PMC5494720.
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Oncol Lett. 2017 Jul;14(1):860-866. doi: 10.3892/ol.2017.6238. Epub 2017 May 24.

RT-qPCR for .

Oncology letters

Victoria Grèze, Justyna Kanold, Fanny Chambon, Pascale Halle, Anne-Sophie Gremeau, Nathalie Rives, Nadège Rouel, Bruno Pereira, Andrei Tchirkov, Florence Brugnon

Affiliations

  1. Service Hématologie Oncologie Pédiatrique, CHU Clermont-Ferrand, France.
  2. Université Clermont Auvergne, INSERM-CIC 1405, Unité CRECHE, CHU Clermont-Ferrand, F-63000 Clermont-Ferrand, France.
  3. Centre de Biothérapie d'Auvergne, CHU Clermont-Ferrand, F-63000 Clermont-Ferrand, France.
  4. Assistance Médicale à la Procréation, CECOS, CHU Clermont-Ferrand, F-63000 Clermont-Ferrand, France.
  5. Normandie Univ, UNIROUEN, EA 4308 'Gamètogenèse et Qualité du Gamète', Rouen University Hospital, F-76000 Rouen, France.
  6. Laboratoire de Biologie de la Reproduction-CECOS, Rouen University Hospital, F-76000 Rouen, France.
  7. Unité de Biostatistiques, Délégation à la Recherche Clinique et l'Innovation, CHU Clermont-Ferrand, F-63000 Clermont Ferrand, France.
  8. Cytogénétique Médicale, CHU Clermont-Ferrand, F-63000 Clermont Ferrand, France.
  9. Université Clermont Auvergne, INSERM, U1240 Imagerie Moléculaire et Stratégies Théranostiques, CHU Clermont-Ferrand, F-63000 Clermont Ferrand, France.

PMID: 28693243 PMCID: PMC5494720 DOI: 10.3892/ol.2017.6238

Abstract

Neuroblastoma (NB) is the most common type of extracranial solid tumor in children with a high prevalence in toddlers. For childhood cancer survivors, preservation of reproductive potential is an important factor for quality of life. The optimization of NB minimal residual disease (MRD) detection in testicular tissue is crucial to evaluate the risk of malignant cell reintroduction. The first step in the present study was to assess the accuracy of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to detect tyrosine hydroxylase (

Keywords: fertility preservation; minimal residual disease; neuroblastoma; reverse transcription quantitative polymerase chain reaction; testicular tissue

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