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Merey HA, Abd-Elmonem MS, Nazlawy HN, et al. Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation. J Anal Methods Chem. 2017;2017:2321572doi: 10.1155/2017/2321572.
Merey, H. A., Abd-Elmonem, M. S., Nazlawy, H. N., & Zaazaa, H. E. (2017). Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation. Journal of analytical methods in chemistry, 20172321572. https://doi.org/10.1155/2017/2321572
Merey, Hanan A, et al. "Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation." Journal of analytical methods in chemistry vol. 2017 (2017): 2321572. doi: https://doi.org/10.1155/2017/2321572
Merey HA, Abd-Elmonem MS, Nazlawy HN, Zaazaa HE. Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation. J Anal Methods Chem. 2017;2017:2321572. doi: 10.1155/2017/2321572. Epub 2017 Jul 24. PMID: 28811956; PMCID: PMC5546081.
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J Anal Methods Chem. 2017;2017:2321572. doi: 10.1155/2017/2321572. Epub 2017 Jul 24.

Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation.

Journal of analytical methods in chemistry

Hanan A Merey, Mahmmoud S Abd-Elmonem, Hagar N Nazlawy, Hala E Zaazaa

Affiliations

  1. Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El Aini, Cairo 11562, Egypt.
  2. National Organization for Drug Control and Research (NODCAR), 6 Abu Hazem Street, Pyramids Ave., P.O. Box 29, Giza, Egypt.

PMID: 28811956 PMCID: PMC5546081 DOI: 10.1155/2017/2321572

Abstract

Four precise, accurate, selective, and sensitive UV-spectrophotometric methods were developed and validated for the simultaneous determination of a binary mixture of Oxytetracycline HCl (OXY) and Flunixin Meglumine (FLU). The first method, dual wavelength (DW), depends on measuring the difference in absorbance (ΔA 273.4-327 nm) for the determination of OXY where FLU is zero while FLU is determined at ΔA 251.7-275.7 nm. The second method, first-derivative spectrophotometric method (1D), depends on measuring the peak amplitude of the first derivative selectively at 377 and 266.7 nm for the determination of OXY and FLU, respectively. The third method, ratio difference method, depends on the difference in amplitudes of the ratio spectra at ΔP 286.5-324.8 nm and ΔP 249.6-286.3 nm for the determination of OXY and FLU, respectively. The fourth method, first derivative of ratio spectra method (1DD), depends on measuring the amplitude peak to peak of the first derivative of ratio spectra at 296.7 to 369 nm and 259.1 to 304.7 nm for the determination of OXY and FLU, respectively. Different factors affecting the applied spectrophotometric methods were studied. The proposed methods were validated according to ICH guidelines. Satisfactory results were obtained for determination of both drugs in laboratory prepared mixture and pharmaceutical dosage form. The developed methods are compared favourably with the official ones.

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