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Subadi I, Nugraha B, Laswati H, et al. Pain Relief with Wet Cupping Therapy in Rats is Mediated by Heat Shock Protein 70 and ß-Endorphin. Iran J Med Sci. 2017;42(4):384-391
Subadi, I., Nugraha, B., Laswati, H., & Josomuljono, H. (2017). Pain Relief with Wet Cupping Therapy in Rats is Mediated by Heat Shock Protein 70 and ß-Endorphin. Iranian journal of medical sciences, 42(4), 384-391.
Subadi, Imam, et al. "Pain Relief with Wet Cupping Therapy in Rats is Mediated by Heat Shock Protein 70 and ß-Endorphin." Iranian journal of medical sciences vol. 42,4 (2017): 384-391.
Subadi I, Nugraha B, Laswati H, Josomuljono H. Pain Relief with Wet Cupping Therapy in Rats is Mediated by Heat Shock Protein 70 and ß-Endorphin. Iran J Med Sci. 2017 Jul;42(4):384-391. PMID: 28761205; PMCID: PMC5523046.
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Iran J Med Sci. 2017 Jul;42(4):384-391.

Pain Relief with Wet Cupping Therapy in Rats is Mediated by Heat Shock Protein 70 and ß-Endorphin.

Iranian journal of medical sciences

Imam Subadi, Boya Nugraha, Hening Laswati, Harjanto Josomuljono

Affiliations

  1. Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Airlangga University, Surabaya, Indonesia.
  2. Department of Rehabilitation Medicine, Hannover Medical School, Hannover, Germany.
  3. Departmentof Physiology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia.

PMID: 28761205 PMCID: PMC5523046

Abstract

BACKGROUND: Wet cupping therapy is a complementary therapy in pain management. The mechanism of this therapy, however, needs further elucidation. Cells injured by wet cupping therapy seem to stimulate the expression of heat shock protein 70 (HSP70). Its benefit in pain reduction could be mediated by the expression of ß-endorphin. This study aimed at determining the correlation between HSP70 and ß-endorphin after wet cupping therapy.

METHODS: Sixteen male Wistar rats were divided into control (CG; n=8) and treatment (TG; n=8) groups. The rats in both groups were injected with complete Freund's adjuvant (CFA) at the footpad. In the TG, wet cupping therapy was done at the left and right paralumbar regions 48 hours after the CFA injection. Twenty-four hours after therapy, the hot plate test was done to assess pain threshold. Thereafter, immunohistochemistry from the skin subjected to wet cupping therapy was conducted for HSP70 and ß-endorphin.

RESULTS: The expression of HSP70 was significantly higher in the keratinocytes of the TG (20.25±3.53; P<0.001) than in the keratinocytes of the CG (10.50±2.44; P<0.001). The expression of ß-endorphin was significantly higher in the keratinocytes of the TG (22.37±3.52; P<0.001) than in the keratinocytes of the CG (5.12±1.72; P<0.001). The results also revealed a high correlation between HSP70 and ß-endorphin (β=0.864; P<0.001). Pain threshold after wet cupping therapy was significantly higher in the TG (22.81±6.34 s; P=0.003) than in the CG (11.78±3.56 s).

CONCLUSIONS: The benefit of wet cupping therapy in terms of pain reduction in rats could be mediated by the expression of HSP70 and ß-endorphin.

Keywords: Complementary therapies; HSP70 heat-shock proteins; Pain; Rat; β-endorphin

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