Display options
Cite
Farhadi A, Haniloo A, Fazaeli A, et al. PCR-based Diagnosis of . Iran J Parasitol. 2017;12(1):56-62
Farhadi, A., Haniloo, A., Fazaeli, A., Moradian, S., & Farhadi, M. (2017). PCR-based Diagnosis of . Iranian journal of parasitology, 12(1), 56-62.
Farhadi, Atieh, et al. "PCR-based Diagnosis of ." Iranian journal of parasitology vol. 12,1 (2017): 56-62.
Farhadi A, Haniloo A, Fazaeli A, Moradian S, Farhadi M. PCR-based Diagnosis of . Iran J Parasitol. 2017 Jan-Mar;12(1):56-62. PMID: 28761461; PMCID: PMC5522699.
Copy Download .nbib Format: NLM
Share it on

Iran J Parasitol. 2017 Jan-Mar;12(1):56-62.

PCR-based Diagnosis of .

Iranian journal of parasitology

Atieh Farhadi, Ali Haniloo, Asghar Fazaeli, Siamak Moradian, Mehdi Farhadi

Affiliations

  1. Dept. of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
  2. Fellowship in Vitreoretinal Surgery, Ophthalmic Research Center, Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

PMID: 28761461 PMCID: PMC5522699

Abstract

BACKGROUND: The diagnosis of ocular toxoplasmosis is mainly based on clinical features. However, ocular fluid testing by PCR may be very helpful for approval or rejection of this etiology. In this study, we utilized a nested-PCR technique, targeting the B1 partial sequence to analyze the aqueous and vitreous samples for evaluating the presence of the

METHODS: Fifty aqueous or vitreous humor samples were obtained from patients with clinical features of ocular toxoplasmosis admitted to ophthalmology hospitals and clinics in Iran, within 2014. The samples were subsequently subjected to DNA extraction and purification. For nested amplification of the

RESULTS: The first-round PCR resulted in the detection of

CONCLUSION: The nested-PCR protocol using the B1 gene, with the high detection power, could be a useful complimentary method to clinical diagnose of ocular toxoplasmosis.

Keywords: Aqueous; Iran; Nested-PCR; Ocular toxoplasmosis; Vitreous

References

  1. J Clin Microbiol. 1989 Aug;27(8):1787-92 - PubMed
  2. J Clin Microbiol. 2009 Jul;47(7):2131-5 - PubMed
  3. Invest Ophthalmol Vis Sci. 2000 Mar;41(3):634-44 - PubMed
  4. Ocul Immunol Inflamm. 2004 Dec;12(4):297-310 - PubMed
  5. Am J Trop Med Hyg. 2010 Mar;82(3):464-5 - PubMed
  6. J Clin Microbiol. 2008 Jun;46(6):1965-7 - PubMed
  7. Clin Microbiol Rev. 2012 Apr;25(2):264-96 - PubMed
  8. Clin Exp Ophthalmol. 2013 Jan-Feb;41(1):95-108 - PubMed
  9. Lancet. 2004 Jun 12;363(9425):1965-76 - PubMed
  10. J Clin Microbiol. 2004 Oct;42(10):4765-8 - PubMed
  11. Int J Ophthalmol. 2011;4(2):199-201 - PubMed
  12. Am J Ophthalmol. 2009 Jan;147(1):140-147.e2 - PubMed
  13. Acta Biomed. 2006 Aug;77(2):75-80 - PubMed
  14. Int J Parasitol. 2000 Jan;30(1):69-75 - PubMed
  15. Clinics (Sao Paulo). 2009;64(3):171-6 - PubMed
  16. Ophthalmology. 1999 Aug;106(8):1554-63 - PubMed
  17. J Clin Microbiol. 2010 Feb;48(2):591-2 - PubMed
  18. Mem Inst Oswaldo Cruz. 2009 Mar;104(2):351-7 - PubMed
  19. Ocul Immunol Inflamm. 2011 Aug;19(4):255-61 - PubMed
  20. J Clin Microbiol. 2012 Apr;50(4):1452-4 - PubMed

Publication Types