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Krętowski R, Kusaczuk M, Naumowicz M, et al. The Effects of Silica Nanoparticles on Apoptosis and Autophagy of Glioblastoma Cell Lines. Nanomaterials (Basel). 2017;7(8)doi: 10.3390/nano7080230.
Krętowski, R., Kusaczuk, M., Naumowicz, M., Kotyńska, J., Szynaka, B., & Cechowska-Pasko, M. (2017). The Effects of Silica Nanoparticles on Apoptosis and Autophagy of Glioblastoma Cell Lines. Nanomaterials (Basel, Switzerland), 7(8), . https://doi.org/10.3390/nano7080230
Krętowski, Rafał, et al. "The Effects of Silica Nanoparticles on Apoptosis and Autophagy of Glioblastoma Cell Lines." Nanomaterials (Basel, Switzerland) vol. 7,8 (2017). doi: https://doi.org/10.3390/nano7080230
Krętowski R, Kusaczuk M, Naumowicz M, Kotyńska J, Szynaka B, Cechowska-Pasko M. The Effects of Silica Nanoparticles on Apoptosis and Autophagy of Glioblastoma Cell Lines. Nanomaterials (Basel). 2017 Aug 21;7(8). doi: 10.3390/nano7080230. PMID: 28825685; PMCID: PMC5575712.
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Nanomaterials (Basel). 2017 Aug 21;7(8). doi: 10.3390/nano7080230.

The Effects of Silica Nanoparticles on Apoptosis and Autophagy of Glioblastoma Cell Lines.

Nanomaterials (Basel, Switzerland)

Rafał Krętowski, Magdalena Kusaczuk, Monika Naumowicz, Joanna Kotyńska, Beata Szynaka, Marzanna Cechowska-Pasko

Affiliations

  1. Department of Pharmaceutical Biochemistry, Medical University of Bia?ystok, Mickiewicza 2A, 15-222 Bia?ystok, Poland. [email protected].
  2. Department of Pharmaceutical Biochemistry, Medical University of Bia?ystok, Mickiewicza 2A, 15-222 Bia?ystok, Poland. [email protected].
  3. Institute of Chemistry, University of Bialystok, K. Cio?kowskiego 1K, 15-245 Bia?ystok, Poland. [email protected].
  4. Institute of Chemistry, University of Bialystok, K. Cio?kowskiego 1K, 15-245 Bia?ystok, Poland. [email protected].
  5. Department of Histology and Embryology, Medical University of Bia?ystok,Waszyngtona 13, 15-269 Bia?ystok, Poland. [email protected].
  6. Department of Pharmaceutical Biochemistry, Medical University of Bia?ystok, Mickiewicza 2A, 15-222 Bia?ystok, Poland. [email protected].

PMID: 28825685 PMCID: PMC5575712 DOI: 10.3390/nano7080230

Abstract

Silica nanoparticles (SiNPs) are one of the most commonly used nanomaterials in various medical applications. However, possible mechanisms of the toxicity caused by SiNPs remain unclear. The study presented here provides novel information on molecular and cellular effects of SiNPs in glioblastoma LBC3 and LN-18 cells. It has been demonstrated that SiNPs of 7 nm, 5-15 nm and 10-20 nm induce time- and dose-dependent cytotoxicity in LBC3 and LN-18 cell lines. In contrast to glioblastoma cells, we observed only weak reduction in viability of normal skin fibroblasts treated with SiNPs. Furthermore, in LBC3 cells treated with 5-15 nm SiNPs we noticed induction of apoptosis and necrosis, while in LN-18 cells only necrosis. The 5-15 nm SiNPs were also found to cause oxidative stress, a loss in mitochondrial membrane potential, and changes in the ultrastructure of the mitochondria in LBC3 cells. Quantitative real-time PCR results showed that in LBC3 cells the mRNA levels of pro-apoptotic genes

Keywords: apoptosis; autophagy; glioblastoma multiforme; nanotoxicity; silica nanoparticles

Conflict of interest statement

The authors report no conflicts of interest in this work.

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