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Velasquez C, Vasquez JS, Balcazar N. In Vitro Effect of Fatty Acids Identified in the Plasma of Obese Adolescents on the Function of Pancreatic β-Cells. Diabetes Metab J. 2017;41(4):303-315doi: 10.4093/dmj.2017.41.4.303.
Velasquez, C., Vasquez, J. S., & Balcazar, N. (2017). In Vitro Effect of Fatty Acids Identified in the Plasma of Obese Adolescents on the Function of Pancreatic β-Cells. Diabetes & metabolism journal, 41(4), 303-315. https://doi.org/10.4093/dmj.2017.41.4.303
Velasquez, Claudia, et al. "In Vitro Effect of Fatty Acids Identified in the Plasma of Obese Adolescents on the Function of Pancreatic β-Cells." Diabetes & metabolism journal vol. 41,4 (2017): 303-315. doi: https://doi.org/10.4093/dmj.2017.41.4.303
Velasquez C, Vasquez JS, Balcazar N. In Vitro Effect of Fatty Acids Identified in the Plasma of Obese Adolescents on the Function of Pancreatic β-Cells. Diabetes Metab J. 2017 Aug;41(4):303-315. doi: 10.4093/dmj.2017.41.4.303. PMID: 28868828; PMCID: PMC5583408.
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Diabetes Metab J. 2017 Aug;41(4):303-315. doi: 10.4093/dmj.2017.41.4.303.

In Vitro Effect of Fatty Acids Identified in the Plasma of Obese Adolescents on the Function of Pancreatic β-Cells.

Diabetes & metabolism journal

Claudia Velasquez, Juan Sebastian Vasquez, Norman Balcazar

Affiliations

  1. Research Group in Food and Human Nutrition, School of Dietetics and Human Nutrition, University of Antioquia, Medellin, Colombia.
  2. Genetics Molecular Group, University of Antioquia, Medellin, Colombia.
  3. Department of Physiology and Biochemistry, School of Medicine, University of Antioquia, Medellin, Colombia. [email protected].

PMID: 28868828 PMCID: PMC5583408 DOI: 10.4093/dmj.2017.41.4.303

Abstract

BACKGROUND: The increase in circulating free fatty acid (FFA) levels is a major factor that induces malfunction in pancreatic β-cells. We evaluated the effect of FFAs reconstituted according to the profile of circulating fatty acids found in obese adolescents on the viability and function of the murine insulinoma cell line (mouse insulinoma [MIN6]).

METHODS: From fatty acids obtained commercially, plasma-FFA profiles of three different youth populations were reconstituted: obese with metabolic syndrome; obese without metabolic syndrome; and normal weight without metabolic syndrome. MIN6 cells were treated for 24 or 48 hours with the three FFA profiles, and glucose-stimulated insulin secretion, cell viability, mitochondrial function and antioxidant activity were evaluated.

RESULTS: The high FFA content and high polyunsaturated ω6/ω3 ratio, present in plasma of obese adolescents with metabolic syndrome had a toxic effect on MIN6 cell viability and function, increasing oxidative stress and decreasing glucose-dependent insulin secretion.

CONCLUSION: These results could help to guide nutritional management of obese young individuals, encouraging the increase of ω-3-rich food consumption in order to reduce the likelihood of deterioration of β-cells and the possible development of type 2 diabetes mellitus.

Copyright © 2017 Korean Diabetes Association

Keywords: Fatty acids, nonesterified; Insulin-secreting cells; Lipotoxicity; Metabolic syndrome; Obesity

Conflict of interest statement

No potential conflict of interest relevant to this article was reported.

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