Display options
Cite
Li Z, Wang W, Wang X, et al. Sustained-released mixture of vascular endothelial growth factor 165 and fibrin glue strengthens healing of ileal anastomoses in a rabbit model with intraperitoneal infection. Ann Surg Treat Res. 2017;93(3):159-165doi: 10.4174/astr.2017.93.3.159.
Li, Z., Wang, W., Wang, X., Jiang, L., Wang, F., & Liu, Q. (2017). Sustained-released mixture of vascular endothelial growth factor 165 and fibrin glue strengthens healing of ileal anastomoses in a rabbit model with intraperitoneal infection. Annals of surgical treatment and research, 93(3), 159-165. https://doi.org/10.4174/astr.2017.93.3.159
Li, Zhanwu, et al. "Sustained-released mixture of vascular endothelial growth factor 165 and fibrin glue strengthens healing of ileal anastomoses in a rabbit model with intraperitoneal infection." Annals of surgical treatment and research vol. 93,3 (2017): 159-165. doi: https://doi.org/10.4174/astr.2017.93.3.159
Li Z, Wang W, Wang X, Jiang L, Wang F, Liu Q. Sustained-released mixture of vascular endothelial growth factor 165 and fibrin glue strengthens healing of ileal anastomoses in a rabbit model with intraperitoneal infection. Ann Surg Treat Res. 2017 Sep;93(3):159-165. doi: 10.4174/astr.2017.93.3.159. Epub 2017 Aug 30. PMID: 28932732; PMCID: PMC5597540.
Copy Download .nbib Format: NLM
Share it on

Ann Surg Treat Res. 2017 Sep;93(3):159-165. doi: 10.4174/astr.2017.93.3.159. Epub 2017 Aug 30.

Sustained-released mixture of vascular endothelial growth factor 165 and fibrin glue strengthens healing of ileal anastomoses in a rabbit model with intraperitoneal infection.

Annals of surgical treatment and research

Zhanwu Li, Wenjun Wang, Xiaozhou Wang, Lei Jiang, Fengyi Wang, Qiang Liu

Affiliations

  1. Department of Emergency Surgery, Affiliated Zhongshan Hospital of Dalian University, Liaoning, China.

PMID: 28932732 PMCID: PMC5597540 DOI: 10.4174/astr.2017.93.3.159

Abstract

PURPOSE: To investigate the effects of a sustained-released mixture of vascular endothelial growth factor 165 (VEGF165) and fibrin glue (FG) local administration on postoperative rabbit ileal anastomoses.

METHODS: One hundred twenty-eight male and female New Zealand white rabbits underwent intraperitoneal infection subsequent ileal anastomosis surgery were divided randomly into 4 groups, including 32 animals in each, applied with saline solution, FG, rhVEGF165 and a mixture of rhVEGF165 with FG (VEGF + FG) on the anastomoses, respectively. The incidences of anastomotic leakage were observed. Histopathological examination for inflammatory infiltration, fibroblast proliferation, and capillary vascular proliferation were performed. Then, bursting pressure and hydroxyproline concentrations were assessed in anastomoses sits on postoperative days 3, 5, 7, and 14.

RESULTS: Rabbits in VEGF + FG group had the lowest incidence of leakage (P < 0.05). Histological evaluations revealed that granulation tissue was formed on days 5 after anastomosis; fibroblast proliferation and capillary vascular proliferation were significantly increased on days 7 and 14 in VEGF + FG group. Furthermore, there was a statistically significant difference in the mean bursting pressures between VEGF + FG group and other groups on days 7 and 14 (P < 0.05), and rabbits in VEGF + FG group exhibited a higher concentration than VEGF group (P < 0.05) and FG group (P < 0.05) on day 14.

CONCLUSION: Administration of VEGF165 mixed with FG to ileal anastomosis accelerates wound healing and enhances the anastomosis by increased angiogenesis.

Keywords: Anastomotic leak; Delayed-action preparations; Fibrin tissue adhesive; Ileum; Vascular endothelial growth factor A

Conflict of interest statement

CONFLICTS OF INTEREST: No potential conflict of interest relevant to this article was reported.

References

  1. Am Heart J. 2001 Nov;142(5):872-80 - PubMed
  2. Thromb Haemost. 2003 Mar;89(3):573-82 - PubMed
  3. Tissue Eng. 2001 Dec;7(6):757-66 - PubMed
  4. J Mol Histol. 2007 Oct;38(5):425-33 - PubMed
  5. Nat Protoc. 2009;4(1):31-6 - PubMed
  6. Ann Surg Treat Res. 2017 Feb;92 (2):97-104 - PubMed
  7. J Artif Organs. 2014 Mar;17(1):42-9 - PubMed
  8. Wound Repair Regen. 2008 Jul-Aug;16(4):542-50 - PubMed
  9. Ann Surg. 1973 May;177(5):513-8 - PubMed
  10. J Mater Sci Mater Med. 2014 Apr;25(4):1165-72 - PubMed
  11. J Vasc Surg. 2000 May;31(5):936-43 - PubMed
  12. Microsurgery. 2006;26(3):131-6 - PubMed
  13. J Vasc Surg. 1995 Feb;21(2):314-24; discussion 324-5 - PubMed
  14. Tech Coloproctol. 2006 Jul;10(2):115-20 - PubMed
  15. J Antimicrob Chemother. 2001 Dec;48(6):861-7 - PubMed
  16. Cells Tissues Organs. 2009;189(5):327-37 - PubMed
  17. Cytotherapy. 2012 May;14(5):555-62 - PubMed
  18. J Control Release. 2010 Nov 20;148(1):49-55 - PubMed
  19. Ann Surg. 2007 Aug;246(2):207-14 - PubMed
  20. Int J Artif Organs. 2003 Feb;26(2):161-9 - PubMed
  21. Plast Reconstr Surg. 2003 Sep 15;112(4):1032-9 - PubMed
  22. Hepatogastroenterology. 2011 May-Jun;58(107-108):922-5 - PubMed
  23. J Control Release. 2010 May 10;143(3):282-9 - PubMed
  24. Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7657-61 - PubMed
  25. Cell Struct Funct. 2001 Feb;26(1):25-35 - PubMed
  26. J Gene Med. 2002 Jul-Aug;4(4):371-80 - PubMed

Publication Types