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Int J Syst Evol Microbiol. 2015 Nov;65(11):3800-3805. doi: 10.1099/ijsem.0.000497.

Bacillus formosensis sp. nov., isolated from pesticide wastewater.

International journal of systematic and evolutionary microbiology

Shih-Yao Lin, Asif Hameed, You-Cheng Liu, Yi-Han Hsu, Wei-An Lai, Wen-Shao Yen, Chiu-Chung Young

Affiliations

  1. Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
  2. Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan, ROC.

PMID: 28875914 DOI: 10.1099/ijsem.0.000497

Abstract

A Gram-stain-positive, endospore-forming rod (designated strain CC-LY275T) was isolated from a pesticide wastewater sample. The isolate grew at a temperature 20-45 °C, at pH 7.0-8.0 and tolerated NaCl 6 % (w/v). The most closely related strains in terms of 16S rRNA gene sequence similarity were Bacillus horneckiae (97.1 %) and Bacillus oceanisediminis (96.8 %), respectively. The G+C content of the genomic DNA was 37.9 mol%. Strain CC-LY275T was determined to possess iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0 as predominant fatty acids. The major polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Cell-wall peptidoglycan contained meso-diaminopimelic acid; menaquinone (MK-7) was the predominant respiratory quinone. According to the distinct phylogenetic, phenotypic and chemotaxonomic properties, the name Bacillus formosensis sp. nov. (type strain CC-LY275T = BCRC 80443T = JCM 18448T) is proposed.

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