Display options
Cite
Popkin DL, Zilka S, Dimaano M, et al. Cetylpyridinium Chloride (CPC) Exhibits Potent, Rapid Activity Against Influenza Viruses . Pathog Immun. 2017;2(2):252-269doi: 10.20411/pai.v2i2.200.
Popkin, D. L., Zilka, S., Dimaano, M., Fujioka, H., Rackley, C., Salata, R., Griffith, A., Mukherjee, P. K., Ghannoum, M. A., & Esper, F. (2017). Cetylpyridinium Chloride (CPC) Exhibits Potent, Rapid Activity Against Influenza Viruses . Pathogens & immunity, 2(2), 252-269. https://doi.org/10.20411/pai.v2i2.200
Popkin, Daniel L, et al. "Cetylpyridinium Chloride (CPC) Exhibits Potent, Rapid Activity Against Influenza Viruses ." Pathogens & immunity vol. 2,2 (2017): 252-269. doi: https://doi.org/10.20411/pai.v2i2.200
Popkin DL, Zilka S, Dimaano M, Fujioka H, Rackley C, Salata R, Griffith A, Mukherjee PK, Ghannoum MA, Esper F. Cetylpyridinium Chloride (CPC) Exhibits Potent, Rapid Activity Against Influenza Viruses . Pathog Immun. 2017;2(2):252-269. doi: 10.20411/pai.v2i2.200. Epub 2017 Jun 26. PMID: 28936484; PMCID: PMC5605151.
Copy Download .nbib Format: NLM
Share it on

Pathog Immun. 2017;2(2):252-269. doi: 10.20411/pai.v2i2.200. Epub 2017 Jun 26.

Cetylpyridinium Chloride (CPC) Exhibits Potent, Rapid Activity Against Influenza Viruses .

Pathogens & immunity

Daniel L Popkin, Sarah Zilka, Matthew Dimaano, Hisashi Fujioka, Cristina Rackley, Robert Salata, Alexis Griffith, Pranab K Mukherjee, Mahmoud A Ghannoum, Frank Esper

Affiliations

  1. Department of Dermatology, University Hospitals Cleveland Medical Center and Case Western Reserve University, Cleveland, Ohio.
  2. Center for Medical Mycology, Department of Dermatology, University Hospitals Cleveland Medical Center and Case Western Reserve University, Cleveland, Ohio.
  3. Department of Medicine, The University of Chicago, Chicago, Illinois.
  4. Electron Microscopy Core Facility, Case Western Reserve University School of Medicine.
  5. Hathaway Brown Science Research and Engineering Program, Cleveland, Ohio.
  6. Division of Infectious Diseases and HIV Medicine, University Hospitals Cleveland Medical Center and Case Western Reserve University, Cleveland, Ohio.
  7. Division of Pediatric Infectious Diseases, University Hospitals Cleveland Medical Center and Case Western Reserve University, Cleveland, Ohio.

PMID: 28936484 PMCID: PMC5605151 DOI: 10.20411/pai.v2i2.200

Abstract

BACKGROUND: There is a continued need for strategies to prevent influenza. While cetylpyridinium chloride (CPC), a broad-spectrum antimicrobial agent, has an extensive antimicrobial spectrum, its ability to affect respiratory viruses has not been studied in detail.

OBJECTIVES: Here, we evaluate the ability of CPC to disrupt influenza viruses

METHODS: The virucidal activity of CPC was evaluated against susceptible and oseltamivir-resistant strains of influenza viruses. The effective virucidal concentration (EC) of CPC was determined using a hemagglutination assay and tissue culture infective dose assay. The effect of CPC on viral envelope morphology and ultrastructure was evaluated using transmission electron microscopy (TEM). The ability of influenza virus to develop resistance was evaluated after multiple passaging in sub-inhibitory concentrations of CPC. Finally, the efficacy of CPC in formulation to prevent and treat influenza infection was evaluated using the PR8 murine influenza model.

RESULTS: The virucidal effect of CPC occurred within 10 minutes, with mean EC

CONCLUSIONS: The antimicrobial agent CPC possesses virucidal activity against susceptible and resistant strains of influenza virus by targeting and disrupting the viral envelope. Substantial virucidal activity is seen even at very low concentrations of CPC without development of resistance. Moreover, CPC in formulation reduces influenza-associated mortality and morbidity

Keywords: CPC; Cetylpyridinium Chloride; Influenza; Respiratory tract illness; quaternary ammonium compound; respiratory virus

Conflict of interest statement

TRANSPARENCY DECLARATIONS MAG acts as Chief Scientific Officer and Consultant for ARMS Pharmaceutical LLC. PKM acts as a Consultant for ARMS Pharmaceutical LLC. There is no conflict of interest for th

References

  1. MMWR Recomm Rep. 2016 Aug 26;65(5):1-54 - PubMed
  2. Vaccine. 2007 Jun 28;25(27):5086-96 - PubMed
  3. Health Technol Assess. 2009 Nov;13(58):1-265, iii-iv - PubMed
  4. J Clin Periodontol. 2008 Jun;35(6):547-54 - PubMed
  5. N Engl J Med. 2008 Dec 11;359(24):2579-85 - PubMed
  6. Cochrane Database Syst Rev. 2014 Mar 13;(3):CD001269 - PubMed
  7. Cochrane Database Syst Rev. 2012 Jan 18;1:CD008965 - PubMed
  8. Lancet. 2009 Oct 17;374(9698):1312-3 - PubMed
  9. J Appl Microbiol. 2005;99(4):703-15 - PubMed
  10. Clin Infect Dis. 2009 Apr 15;48(8):1003-32 - PubMed
  11. Virology. 2011 Mar 30;412(1):36-45 - PubMed
  12. Antimicrob Agents Chemother. 2009 Oct;53(10):4433-40 - PubMed
  13. Pathog Immun. 2017;2(2):252-269 - PubMed
  14. BMJ. 2013 Jul 23;347:f4656 - PubMed
  15. Infect Dis Clin North Am. 2010 Jun;24(2):413-37 - PubMed
  16. J Virol. 2014 Jan;88(1):272-81 - PubMed
  17. Curr Protoc Microbiol. 2006 Dec;Chapter 15:Unit 15G.1 - PubMed
  18. Influenza Other Respir Viruses. 2013 Jan;7 Suppl 1:25-36 - PubMed
  19. Lancet Infect Dis. 2009 Aug;9(8):493-504 - PubMed
  20. J Med Microbiol. 1995 Jun;42(6):415-20 - PubMed
  21. Br Med Bull. 2002;61:215-30 - PubMed
  22. MMWR Surveill Summ. 2013 Oct 25;62(4):1-28 - PubMed
  23. MMWR Morb Mortal Wkly Rep. 2014 Feb 21;63(7):137-42 - PubMed
  24. ACS Infect Dis. 2015 Sep 11;1(9):399-452 - PubMed
  25. Nat Protoc. 2014 Nov;9(11):2663-81 - PubMed
  26. Arzneimittelforschung. 2001;51(7):588-95 - PubMed
  27. Clin Microbiol Rev. 2013 Jan;26(1):135-62 - PubMed
  28. MMWR Recomm Rep. 2011 Jan 21;60(1):1-24 - PubMed
  29. J Infect Dis. 2011 Jan 1;203(1):25-31 - PubMed
  30. Lancet. 2013 Jun 29;381(9885):2273-9 - PubMed
  31. J Appl Microbiol. 2002;92 Suppl:16S-27S - PubMed
  32. Nat Protoc. 2008;3(7):1125-31 - PubMed
  33. Int J Mol Sci. 2013 May 10;14(5):9906-46 - PubMed
  34. PLoS One. 2013;8(4):e60348 - PubMed
  35. PLoS Pathog. 2013 Jan;9(1):e1003057 - PubMed
  36. Pediatrics. 2015 Oct;136(4):792-808 - PubMed
  37. BMC Infect Dis. 2017 Jan 14;17(1):74 - PubMed

Publication Types

Grant support