Display options
Cite
Wang C, He H, Zhang JL, et al. High performance liquid chromatography (HPLC) fingerprints and primary structure identification of corn peptides by HPLC-diode array detection and HPLC-electrospray ionization tandem mass spectrometry. J Food Drug Anal. 2015;24(1):95-104doi: 10.1016/j.jfda.2015.05.005.
Wang, C., He, H., Zhang, J. L., Li, X., & Ma, Z. L. (2016). High performance liquid chromatography (HPLC) fingerprints and primary structure identification of corn peptides by HPLC-diode array detection and HPLC-electrospray ionization tandem mass spectrometry. Journal of food and drug analysis, 24(1), 95-104. https://doi.org/10.1016/j.jfda.2015.05.005
Wang, Chi, et al. "High performance liquid chromatography (HPLC) fingerprints and primary structure identification of corn peptides by HPLC-diode array detection and HPLC-electrospray ionization tandem mass spectrometry." Journal of food and drug analysis vol. 24,1 (2016): 95-104. doi: https://doi.org/10.1016/j.jfda.2015.05.005
Wang C, He H, Zhang JL, Li X, Ma ZL. High performance liquid chromatography (HPLC) fingerprints and primary structure identification of corn peptides by HPLC-diode array detection and HPLC-electrospray ionization tandem mass spectrometry. J Food Drug Anal. 2016 Jan;24(1):95-104. doi: 10.1016/j.jfda.2015.05.005. Epub 2015 Jul 22. PMID: 28911415.
Copy Download .nbib Format: NLM
Share it on

J Food Drug Anal. 2016 Jan;24(1):95-104. doi: 10.1016/j.jfda.2015.05.005. Epub 2015 Jul 22.

High performance liquid chromatography (HPLC) fingerprints and primary structure identification of corn peptides by HPLC-diode array detection and HPLC-electrospray ionization tandem mass spectrometry.

Journal of food and drug analysis

Chi Wang, Hui He, Jiu-Liang Zhang, Xing Li, Zhi-Li Ma

Affiliations

  1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan, China.
  2. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan, China. Electronic address: [email protected].

PMID: 28911415 DOI: 10.1016/j.jfda.2015.05.005

Abstract

Corn peptides (CPs) are reported to have many biological functions, such as facilitating alcohol metabolism, antioxidation, antitumor, antihypertension, and hepatoprotection. To develop a method for quality control, the high-performance liquid chromatography (HPLC) system was applied. Twenty-eight common peaks were found in all the CPs of corn samples from Enshi, China, based on which, a fingerprinting chromatogram was established for use in quality control in future research. Subsequently, the major chemical constituents of these common peaks were identified respectively using the HPLC-diode-array detection electrospray ionization tandem mass spectrometry (DAD-ESI-MS/MS) system, and 48 peptide fractions were determined ultimately. This was the first time for the majority of these peptides to be reported, and many of them contained amino acids of glutamine (Q), L and A, which might play an important role in the exhibition of the bioactivities of CPs. Many peptides had a similar primary structure to the peptides which had been proven to be bioactive such as facilitating alcohol metabolism, scavenging free radicals, and inhibiting lipid peroxidation. This systematical analysis of the primary structure of CPs facilitated subsequent studies on the relationship between the structures and functions, and could accelerate holistic research on CPs.

Copyright © 2015. Published by Elsevier B.V.

Keywords: HPLC fingerprints; corn peptides; electrospray ionization mass spectrometry (ESI-MS); high-performance liquid chromatography (HPLC); primary structure

Publication Types