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J Food Drug Anal. 2014 Sep;22(3):296-302. doi: 10.1016/j.jfda.2013.11.001. Epub 2013 Dec 18.

Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica.

Journal of food and drug analysis

Quy Diem Do, Artik Elisa Angkawijaya, Phuong Lan Tran-Nguyen, Lien Huong Huynh, Felycia Edi Soetaredjo, Suryadi Ismadji, Yi-Hsu Ju

Affiliations

  1. Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106-07, Taiwan.
  2. Department of Chemical Engineering, Can Tho University, Can Tho City, Viet Nam.
  3. Department of Chemical Engineering, Widya Mandala Surabaya Catholic University, Kalijudan 37, Surabaya 60114, Indonesia.
  4. Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106-07, Taiwan. Electronic address: [email protected].

PMID: 28911418 DOI: 10.1016/j.jfda.2013.11.001

Abstract

Limnophila aromatica is commonly used as a spice and a medicinal herb in Southeast Asia. In this study, water and various concentrations (50%, 75%, and 100%) of methanol, ethanol, and acetone in water were used as solvent in the extraction of L. aromatica. The antioxidant activity, total phenolic content, and total flavonoid content of the freeze-dried L. aromatica extracts were investigated using various in vitro assays. The extract obtained by 100% ethanol showed the highest total antioxidant activity, reducing power and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The same extract also exhibited the highest phenolic content (40.5 mg gallic acid equivalent/g of defatted L. aromatica) and the highest flavonoid content (31.11 mg quercetin equivalent/g of defatted L. aromatica). The highest extraction yield was obtained by using 50% aqueous acetone. These results indicate that L. aromatica can be used in dietary applications with a potential to reduce oxidative stress.

Copyright © 2013. Published by Elsevier B.V.

Keywords: Antioxidants activity; Effect of solvent; Food analysis; Limnophila aromatica; Total phenol content

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