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Ismail R, Lee HY, Mahyudin NA, et al. Linearity study on detection and quantification limits for the determination of avermectins using linear regression. J Food Drug Anal. 2014;22(4):407-412doi: 10.1016/j.jfda.2014.01.026.
Ismail, R., Lee, H. Y., Mahyudin, N. A., & Abu Bakar, F. (2014). Linearity study on detection and quantification limits for the determination of avermectins using linear regression. Journal of food and drug analysis, 22(4), 407-412. https://doi.org/10.1016/j.jfda.2014.01.026
Ismail, Rafidah, et al. "Linearity study on detection and quantification limits for the determination of avermectins using linear regression." Journal of food and drug analysis vol. 22,4 (2014): 407-412. doi: https://doi.org/10.1016/j.jfda.2014.01.026
Ismail R, Lee HY, Mahyudin NA, Abu Bakar F. Linearity study on detection and quantification limits for the determination of avermectins using linear regression. J Food Drug Anal. 2014 Dec;22(4):407-412. doi: 10.1016/j.jfda.2014.01.026. Epub 2014 May 06. PMID: 28911453.
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J Food Drug Anal. 2014 Dec;22(4):407-412. doi: 10.1016/j.jfda.2014.01.026. Epub 2014 May 06.

Linearity study on detection and quantification limits for the determination of avermectins using linear regression.

Journal of food and drug analysis

Rafidah Ismail, Hai Yen Lee, Nor Ainy Mahyudin, Fatimah Abu Bakar

Affiliations

  1. National Public Health Laboratory, Ministry of Health Malaysia, Lot 1853 Kampung Melayu, 47000 Sg Buloh, Selangor Darul Ehsan, Malaysia; Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia; Food Safety Research Centre (FOSREC), Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
  2. Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia; Food Safety Research Centre (FOSREC), Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
  3. Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia; Food Safety Research Centre (FOSREC), Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia. Electronic address: [email protected].

PMID: 28911453 DOI: 10.1016/j.jfda.2014.01.026

Abstract

In this study, linear relationships between response and concentration were used to estimate the detection limit (DL) and quantification limit (QL) for five avermectins: emamectin, abamectin, doramectin, moxidectin, and ivermectin. Estimation of DL and QL was based on the standard deviation of residual and y-intercept of the regression line at low concentrations of avermectins, using the dispersive solid-phase extraction procedure. Avermectin extracts were analyzed using liquid chromatography tandem mass spectrometry. Based on the regression slope, DL and QL were higher at concentrations of 0.3-0.4 μg/kg and 1 μg/kg, respectively, for all avermectin compounds. Linearity assessment was performed by linear regression, which incorporated a regression model, outlier rejection, and evaluation of the assumption with a significant test. For all avermectins, there is a significant correlation between response and concentration in the range 1-15 μg/kg, and the y-intercept passes through origin (zero).

Copyright © 2014. Published by Elsevier B.V.

Keywords: Avermectins; Detection limit; Dispersive solid-phase extraction; Linear regression; Linearity; Quantification limit

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