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Montero-Mendieta S, Grabherr M, Lantz H, et al. A practical guide to build . PeerJ. 2017;5:e3702doi: 10.7717/peerj.3702.
Montero-Mendieta, S., Grabherr, M., Lantz, H., De la Riva, I., Leonard, J. A., Webster, M. T., & Vilà, C. (2017). A practical guide to build . PeerJ, 5e3702. https://doi.org/10.7717/peerj.3702
Montero-Mendieta, Santiago, et al. "A practical guide to build ." PeerJ vol. 5 (2017): e3702. doi: https://doi.org/10.7717/peerj.3702
Montero-Mendieta S, Grabherr M, Lantz H, De la Riva I, Leonard JA, Webster MT, Vilà C. A practical guide to build . PeerJ. 2017 Sep 01;5:e3702. doi: 10.7717/peerj.3702. eCollection 2017. PMID: 28879061; PMCID: PMC5582611.
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PeerJ. 2017 Sep 01;5:e3702. doi: 10.7717/peerj.3702. eCollection 2017.

A practical guide to build .

PeerJ

Santiago Montero-Mendieta, Manfred Grabherr, Henrik Lantz, Ignacio De la Riva, Jennifer A Leonard, Matthew T Webster, Carles Vilà

Affiliations

  1. Conservation and Evolutionary Genetics Group, Department of Integrative Ecology, Doñana Biological Station (EBD-CSIC), Consejo Superior de Investigaciones Científicas, Seville, Spain.
  2. Department of Medical Biochemistry and Microbiology, National Bioinformatics Infrastructure Sweden (BILS), Uppsala Universitet, Uppsala, Sweden.
  3. Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
  4. Department of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala Universitet, Uppsala, Sweden.

PMID: 28879061 PMCID: PMC5582611 DOI: 10.7717/peerj.3702

Abstract

Whole genome sequencing (WGS) is a very valuable resource to understand the evolutionary history of poorly known species. However, in organisms with large genomes, as most amphibians, WGS is still excessively challenging and transcriptome sequencing (RNA-seq) represents a cost-effective tool to explore genome-wide variability. Non-model organisms do not usually have a reference genome and the transcriptome must be assembled

Keywords: Clusters of Orthologous Groups; Frog transcriptome; Gene ontology; Genomics; Kyoto encyclopedia of genes and genomes; Protein domain identification; RNA-seq; Transcriptomics; Trinity

Conflict of interest statement

The authors declare there are no competing interests.

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