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Barrêre B, Driguez PA, Maudrin J, et al. A novel synthetic reversible inhibitor of sialidase efficiently blocks secondary but not primary influenza virus infection of MDCK cells in culture. Arch Virol. 1997;142(7):1365-1380doi: 10.1007/s007050050166.
Barrêre, B., Driguez, P. A., Maudrin, J., Doutheau, A., Aymard, M., & Quash, G. (1997). A novel synthetic reversible inhibitor of sialidase efficiently blocks secondary but not primary influenza virus infection of MDCK cells in culture. Archives of virology, 142(7), 1365-1380. https://doi.org/10.1007/s007050050166
Barrêre, B, et al. "A novel synthetic reversible inhibitor of sialidase efficiently blocks secondary but not primary influenza virus infection of MDCK cells in culture." Archives of virology vol. 142,7 (1997): 1365-1380. doi: https://doi.org/10.1007/s007050050166
Barrêre B, Driguez PA, Maudrin J, Doutheau A, Aymard M, Quash G. A novel synthetic reversible inhibitor of sialidase efficiently blocks secondary but not primary influenza virus infection of MDCK cells in culture. Arch Virol. 1997 Jul;142(7):1365-1380. doi: 10.1007/s007050050166. PMID: 28879398.
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Arch Virol. 1997 Jul;142(7):1365-1380. doi: 10.1007/s007050050166.

A novel synthetic reversible inhibitor of sialidase efficiently blocks secondary but not primary influenza virus infection of MDCK cells in culture.

Archives of virology

B Barrêre, P A Driguez, J Maudrin, A Doutheau, M Aymard, G Quash

Affiliations

  1. Laboratoire d'Immunochimie, Faculté de Médecine Lyon-Sud, Oullins, France, France.
  2. Laboratoire de Chimie Organique, INSA, Villeurbanne, France, and, France.
  3. Laboratoirede Virologie et Bactériologie, UFR de Médecine, Lyon, France, France.

PMID: 28879398 DOI: 10.1007/s007050050166

Abstract

The sodium salts of 2-difluoromethyl-phenyl-α-ketoside of N-acetyl-neuraminic acid (compound 1) and of 4-difluoromethyl-2-methoxy-phenyl-α-ketoside of N-acetylneuraminic acid (compound 2) were designed as potential mechanism-based inhibitors of sialidase. In vitro both of these compounds competitively inhibited the sialidases of Clostridium perfringens and of influenza virus A/HK/1/68. Inhibition was irreversible with the sialidase of Clostridium perfringens whereas it was reversible with that of A/HK/1/68. Compound 2 did not inhibit the hemagglutinin of the virus but exhibited significant anti-influenza activity when added to the medium of Madin-Darby canine kidney (MDCK) cells infected by influenza virus. In non-infected MDCK cells no inhibition of cellular sialidase was observed. Compound 2 did not block primary infection, but inhibited the release of progeny virus from infected cells. Even after 8 passages in its presence, no resistant strains were detected. Because of its high Ki (8 × 10

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