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Patricia KA, Hoti SL, Kanungo R, et al. Improving the Diagnosis of Scrub Typhus by Combining . J Clin Diagn Res. 2017;11(8):DC27-DC31doi: 10.7860/JCDR/2017/26523.10519.
Patricia, K. A., Hoti, S. L., Kanungo, R., Jambulingam, P., Shashikala, N., & Naik, A. C. (2017). Improving the Diagnosis of Scrub Typhus by Combining . Journal of clinical and diagnostic research : JCDR, 11(8), DC27-DC31. https://doi.org/10.7860/JCDR/2017/26523.10519
Patricia, Karthikeyan Anitha, et al. "Improving the Diagnosis of Scrub Typhus by Combining ." Journal of clinical and diagnostic research : JCDR vol. 11,8 (2017): DC27-DC31. doi: https://doi.org/10.7860/JCDR/2017/26523.10519
Patricia KA, Hoti SL, Kanungo R, Jambulingam P, Shashikala N, Naik AC. Improving the Diagnosis of Scrub Typhus by Combining . J Clin Diagn Res. 2017 Aug;11(8):DC27-DC31. doi: 10.7860/JCDR/2017/26523.10519. Epub 2017 Aug 01. PMID: 28969124; PMCID: PMC5620764.
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J Clin Diagn Res. 2017 Aug;11(8):DC27-DC31. doi: 10.7860/JCDR/2017/26523.10519. Epub 2017 Aug 01.

Improving the Diagnosis of Scrub Typhus by Combining .

Journal of clinical and diagnostic research : JCDR

Karthikeyan Anitha Patricia, Sugeerappa Laxmanappa Hoti, Reba Kanungo, Purushothaman Jambulingam, Nair Shashikala, Ashok C Naik

Affiliations

  1. Research Scholar, Department of Microbiology and Immunology, Vector Control Research Centre, Puducherry, India.
  2. Scientist 'G', Department of Microbiology, Regional Medical Research Centre, Belagavi, Karnataka, India.
  3. Professor, Department of Microbiology, Pondicherry Institute of Medical Sciences, Puducherry, India.
  4. Scientist 'G' and Director, Department of Vector Biology, Vector Control Research Centre, Puducherry, India.
  5. Senior Technical officer, Department of Statistics, Regional Medical Research Centre, Belagavi, Karnataka, India.

PMID: 28969124 PMCID: PMC5620764 DOI: 10.7860/JCDR/2017/26523.10519

Abstract

INTRODUCTION: Scrub typhus, an acute febrile illness, caused by

AIM: The aim of the study was to compare four different methods of detection of scrub typhus and to identify one single test or a combination of tests detecting maximum number of cases.

MATERIALS AND METHODS: One hundred and forty-five suspected scrub typhus cases were included in this study. Duration of fever and presence of eschar in each patient was noted down. Enzyme-Linked Immunosorbent Assay (ELISA) to detect Immunoglobulin M (IgM) antibodies and Polymerase Chain Reaction (PCR) to detect three genes of

RESULTS: These tests showed that IgM ELISA (93%) and PCR (68%) picked up maximum number of positives. Statistical analysis performed using Chi-square test between the diagnostic assays showed that the p - value <0.001 was significant for IgM ELISA. Among the molecular markers, p-value was significant (<0.001) for

CONCLUSION: This study suggests that IgM detection by ELISA and conventional

Keywords: Fever; Laboratory diagnosis; Orientia tsutsugamushi; Rickettsial infections

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