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Nambi Krishnan J, Park SH, Kim SK. Aptamer-Based Single-Step Assay by the Fluorescence Enhancement on Electroless Plated Nano Au Substrate. Sensors (Basel). 2017;17(9)doi: 10.3390/s17092044.
Nambi Krishnan, J., Park, S. H., & Kim, S. K. (2017). Aptamer-Based Single-Step Assay by the Fluorescence Enhancement on Electroless Plated Nano Au Substrate. Sensors (Basel, Switzerland), 17(9), . https://doi.org/10.3390/s17092044
Nambi Krishnan, Jegatha, et al. "Aptamer-Based Single-Step Assay by the Fluorescence Enhancement on Electroless Plated Nano Au Substrate." Sensors (Basel, Switzerland) vol. 17,9 (2017). doi: https://doi.org/10.3390/s17092044
Nambi Krishnan J, Park SH, Kim SK. Aptamer-Based Single-Step Assay by the Fluorescence Enhancement on Electroless Plated Nano Au Substrate. Sensors (Basel). 2017 Sep 07;17(9). doi: 10.3390/s17092044. PMID: 28880198; PMCID: PMC5620733.
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Sensors (Basel). 2017 Sep 07;17(9). doi: 10.3390/s17092044.

Aptamer-Based Single-Step Assay by the Fluorescence Enhancement on Electroless Plated Nano Au Substrate.

Sensors (Basel, Switzerland)

Jegatha Nambi Krishnan, Sang-Hwi Park, Sang Kyung Kim

Affiliations

  1. Center for BioMicrosystems, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Sungbuk-gu, Seoul 136-791, Korea. [email protected].
  2. School of Micro Nano System Engineering, Korea University of Science and Technology, Daejeon 305-333, Korea. [email protected].
  3. School of Chemical Engineering, Birla Institute of Technology and Science, Pilani, K. K. Birla Goa Campus, Zuari Nagar, Goa 403-726, India. [email protected].
  4. Center for BioMicrosystems, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Sungbuk-gu, Seoul 136-791, Korea. [email protected].
  5. School of Micro Nano System Engineering, Korea University of Science and Technology, Daejeon 305-333, Korea. [email protected].
  6. Center for BioMicrosystems, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Sungbuk-gu, Seoul 136-791, Korea. [email protected].
  7. School of Micro Nano System Engineering, Korea University of Science and Technology, Daejeon 305-333, Korea. [email protected].

PMID: 28880198 PMCID: PMC5620733 DOI: 10.3390/s17092044

Abstract

A new single-step aptamer-based surface-enhanced fluorescent optical sensor is built, by combining an aptamer-target interaction for target recognition and a fluorophore interaction for signal enhancement. The developed aptasensor is simple, sensitive, specific and stable for the detection of thrombin. A new nanometallic Au structure in the range of 100 nm was constructed through effective electroless plating method on a Cu thin film. Cu⁺ ions act as sacrificial seeds for the reduction of Au

Keywords: aptamer; electroless deposition; fluorescence; gold nanostructure; human serum albumin; optical biosensor; thrombin

Conflict of interest statement

The authors declare no conflict of interest.

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