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Kawano S, Torisu T, Esaki M, et al. Autophagy promotes degradation of internalized collagen and regulates distribution of focal adhesions to suppress cell adhesion. Biol Open. 2017;6(11):1644-1653doi: 10.1242/bio.027458.
Kawano, S., Torisu, T., Esaki, M., Torisu, K., Matsuno, Y., & Kitazono, T. (2017). Autophagy promotes degradation of internalized collagen and regulates distribution of focal adhesions to suppress cell adhesion. Biology open, 6(11), 1644-1653. https://doi.org/10.1242/bio.027458
Kawano, Shinichi, et al. "Autophagy promotes degradation of internalized collagen and regulates distribution of focal adhesions to suppress cell adhesion." Biology open vol. 6,11 (2017): 1644-1653. doi: https://doi.org/10.1242/bio.027458
Kawano S, Torisu T, Esaki M, Torisu K, Matsuno Y, Kitazono T. Autophagy promotes degradation of internalized collagen and regulates distribution of focal adhesions to suppress cell adhesion. Biol Open. 2017 Nov 15;6(11):1644-1653. doi: 10.1242/bio.027458. PMID: 28970230; PMCID: PMC5703610.
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Biol Open. 2017 Nov 15;6(11):1644-1653. doi: 10.1242/bio.027458.

Autophagy promotes degradation of internalized collagen and regulates distribution of focal adhesions to suppress cell adhesion.

Biology open

Shinichi Kawano, Takehiro Torisu, Motohiro Esaki, Kumiko Torisu, Yuichi Matsuno, Takanari Kitazono

Affiliations

  1. Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
  2. Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan [email protected].

PMID: 28970230 PMCID: PMC5703610 DOI: 10.1242/bio.027458

Abstract

Adhesion of cells to the extracellular matrix (ECM) via focal adhesions (FAs) is crucial for cell survival, migration, and differentiation. Although the regulation of FAs, including by integrins and the ECM, is important to cell behavior, how FAs are regulated is not well known. Autophagy is induced by both cell adhesion and cell detachment. Here, we showed that autophagosomes are located close to internalized collagen and paxillin, which is a well-known marker of FAs. Autophagy-deficient cells showed increased levels of internalized collagen compared with control cells. Moreover, paxillin exhibited a more peripheral distribution and the area of paxillin was increased, and adhesion-induced focal adhesion kinase signaling was impaired and adhesion was enhanced, in autophagy-deficient cells. These results suggest that autophagy suppressed cell adhesion by regulating internalized ECM and FAs.

© 2017. Published by The Company of Biologists Ltd.

Keywords: Atg5; Atg7; Extracellular matrix

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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