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Lan X, Fu LJ, Zhang J, et al. Bisphenol A exposure promotes HTR-8/SVneo cell migration and impairs mouse placentation involving upregulation of integrin-β1 and MMP-9 and stimulation of MAPK and PI3K signaling pathways. Oncotarget. 2017;8(31):51507-51521doi: 10.18632/oncotarget.17882.
Lan, X., Fu, L. J., Zhang, J., Liu, X. Q., Zhang, H. J., Zhang, X., Ma, M. F., Chen, X. M., He, J. L., Li, L. B., Wang, Y. X., & Ding, Y. B. (2017). Bisphenol A exposure promotes HTR-8/SVneo cell migration and impairs mouse placentation involving upregulation of integrin-β1 and MMP-9 and stimulation of MAPK and PI3K signaling pathways. Oncotarget, 8(31), 51507-51521. https://doi.org/10.18632/oncotarget.17882
Lan, Xi, et al. "Bisphenol A exposure promotes HTR-8/SVneo cell migration and impairs mouse placentation involving upregulation of integrin-β1 and MMP-9 and stimulation of MAPK and PI3K signaling pathways." Oncotarget vol. 8,31 (2017): 51507-51521. doi: https://doi.org/10.18632/oncotarget.17882
Lan X, Fu LJ, Zhang J, Liu XQ, Zhang HJ, Zhang X, Ma MF, Chen XM, He JL, Li LB, Wang YX, Ding YB. Bisphenol A exposure promotes HTR-8/SVneo cell migration and impairs mouse placentation involving upregulation of integrin-β1 and MMP-9 and stimulation of MAPK and PI3K signaling pathways. Oncotarget. 2017 May 16;8(31):51507-51521. doi: 10.18632/oncotarget.17882. eCollection 2017 Aug 01. PMID: 28881663; PMCID: PMC5584264.
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Oncotarget. 2017 May 16;8(31):51507-51521. doi: 10.18632/oncotarget.17882. eCollection 2017 Aug 01.

Bisphenol A exposure promotes HTR-8/SVneo cell migration and impairs mouse placentation involving upregulation of integrin-β1 and MMP-9 and stimulation of MAPK and PI3K signaling pathways.

Oncotarget

Xi Lan, Li-Juan Fu, Jun Zhang, Xue-Qing Liu, Hui-Jie Zhang, Xue Zhang, Ming-Fu Ma, Xue-Mei Chen, Jun-Lin He, Lian-Bing Li, Ying-Xiong Wang, Yu-Bin Ding

Affiliations

  1. Department of Reproductive Biology, School of Public Health, Chongqing Medical University, Chongqing, 400016, P.R. China.
  2. Department of Immunology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, 15260, USA.
  3. Center of Molecular Diagnostic Medicine, Life Science Institute, Chongqing Medical University, Chongqing, 400016, P.R. China.
  4. Ministry of Education Key Laboratory of Diagnostic Medicine, College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, P.R. China.
  5. The Key Laboratory of Birth Defects and Reproductive Health of the National Health and Family Planning Commission, Chongqing Population and Family Planning Science and Technology Research Institute, Chongqing, 401147, P.R. China.

PMID: 28881663 PMCID: PMC5584264 DOI: 10.18632/oncotarget.17882

Abstract

In this study, we investigated the effect of Bisphenol A (BPA), an endocrine-disrupting chemical, on the migration of human trophoblasts and mouse placentation by using the primary extravillous trophoblast (EVT) and its cell line HTR-8/SVneo, villous explant cultures, and pregnant mice. BPA increased EVT motility and the outgrowth of villous explants in a dose-dependent manner. BPA also increased the protein levels of integrin-β1 and matrix metalloproteinase (MMP)-9 in human EVTs. Low-dose BPA (≤50 mg) increased the protein levels of MMP-9 and MMP-2 as well as integrin-β1 and integrin-α5 in mouse placenta and decreased the proportion of the labyrinth and spongiotrophoblast layers. Inhibitors of mitogen-activated protein kinase (MAPK) U0126 and phosphatidylinositol-3-kinases (PI3K) LY294002 reversed the protein levels of integrin-β1 and MMP-9 as well as the migratory ability induced by BPA. In conclusion, these results indicated that BPA can enhance trophoblast migration and impair placentation in mice by a mechanism involving upregulation of integrin(s) and MMP(s) as well as the stimulation of MAPK and PI3K/Akt (protein kinase B) signaling pathways.

Keywords: MAPK/PI3K signaling pathway; bisphenol A; cell migration; extravillous trophoblasts; placentation

Conflict of interest statement

CONFLICTS OF INTEREST The authors declare that they have no conflict of interest.

References

  1. Ann N Y Acad Sci. 2003 May;995:84-93 - PubMed
  2. Toxicol Appl Pharmacol. 2009 Dec 15;241(3):322-8 - PubMed
  3. Curr Biol. 2003 Apr 1;13(7):546-53 - PubMed
  4. Chem Res Toxicol. 2015 Sep 21;28(9):1693-703 - PubMed
  5. Toxicol Lett. 2014 Sep 2;229(2):357-65 - PubMed
  6. Environ Health Perspect. 2013 Jan;121(1):138-44 - PubMed
  7. PLoS Genet. 2007 Jan 12;3(1):e5 - PubMed
  8. Reprod Toxicol. 2007 Aug-Sep;24(2):139-77 - PubMed
  9. Environ Health Perspect. 2002 Jun;110 Suppl 3:409-14 - PubMed
  10. Mol Cell Endocrinol. 2006 Jul 25;254-255:179-86 - PubMed
  11. Placenta. 2011 Mar;32(3):241-6 - PubMed
  12. Oncotarget. 2012 Aug;3(8):759-73 - PubMed
  13. Rev Obstet Gynecol. 2012;5(3-4):e137-43 - PubMed
  14. Placenta. 2009 Mar;30(3):284-91 - PubMed
  15. Basic Clin Pharmacol Toxicol. 2016 Oct;119(4):389-95 - PubMed
  16. J Appl Toxicol. 2015 Nov;35(11):1278-91 - PubMed
  17. Curr Protoc Immunol. 2012 Apr;Chapter 7:Unit 7.40.1-11 - PubMed
  18. Mol Cell Endocrinol. 2016 Feb 15;422:74-83 - PubMed
  19. Mol Cell Endocrinol. 2016 Jul 5;429:41-9 - PubMed
  20. Gynecol Obstet Invest. 2007;64(3):126-30 - PubMed
  21. Placenta. 2005 Apr;26 Suppl A:S96-S103 - PubMed
  22. Endocrine. 2002 Oct;19(1):3-11 - PubMed
  23. J Cell Mol Med. 2009 Aug;13(8B):2224-35 - PubMed
  24. Biomed Pharmacother. 2014 Oct;68(8):1037-43 - PubMed
  25. Exp Cell Res. 1993 Jun;206(2):204-11 - PubMed
  26. Physiology (Bethesda). 2005 Jun;20:180-93 - PubMed
  27. Nat Rev Mol Cell Biol. 2009 Dec;10(12):843-53 - PubMed
  28. Environ Toxicol. 2016 Jul;31(7):799-807 - PubMed
  29. Endocrinology. 2011 Mar;152(3):1101-11 - PubMed
  30. Placenta. 2011 Sep;32(9):665-70 - PubMed
  31. Genom Data. 2015 Apr 11;4:109-11 - PubMed
  32. Reprod Toxicol. 2010 Aug;30(1):131-7 - PubMed
  33. Springerplus. 2013 Dec;2(1):54 - PubMed
  34. Hum Reprod. 2010 Apr;25(4):862-73 - PubMed
  35. Environ Health Perspect. 2010 Aug;118(8):1055-70 - PubMed
  36. J Reprod Dev. 2007 Jun;53(3):509-14 - PubMed
  37. Int J Clin Exp Pathol. 2015 Nov 01;8(11):14355-64 - PubMed
  38. Mol Cell Endocrinol. 2015 Sep 5;412:56-64 - PubMed
  39. Arch Biochem Biophys. 2015 May 1;573:52-8 - PubMed
  40. Reprod Toxicol. 2015 Jun;53:39-44 - PubMed
  41. J Prev Med Public Health. 2005 Aug;38(3):330-6 - PubMed
  42. Cell Adh Migr. 2014;8(2):94-7 - PubMed
  43. Mol Cancer. 2009 Nov 24;8:110 - PubMed
  44. Dev Biol. 2002 Oct 15;250(2):358-73 - PubMed
  45. J Cell Sci. 2012 Feb 15;125(Pt 4):943-55 - PubMed
  46. Reprod Toxicol. 2011 Jul;32(1):69-76 - PubMed
  47. PLoS Genet. 2013 Apr;9(4):e1003401 - PubMed
  48. Fertil Steril. 2016 Sep 15;106(4):827-56 - PubMed

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