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Li C, Plamont MA, Sladitschek HL, et al. Dynamic multicolor protein labeling in living cells. Chem Sci. 2017;8(8):5598-5605doi: 10.1039/c7sc01364g.
Li, C., Plamont, M. A., Sladitschek, H. L., Rodrigues, V., Aujard, I., Neveu, P., Le Saux, T., Jullien, L., & Gautier, A. (2017). Dynamic multicolor protein labeling in living cells. Chemical science, 8(8), 5598-5605. https://doi.org/10.1039/c7sc01364g
Li, Chenge, et al. "Dynamic multicolor protein labeling in living cells." Chemical science vol. 8,8 (2017): 5598-5605. doi: https://doi.org/10.1039/c7sc01364g
Li C, Plamont MA, Sladitschek HL, Rodrigues V, Aujard I, Neveu P, Le Saux T, Jullien L, Gautier A. Dynamic multicolor protein labeling in living cells. Chem Sci. 2017 Aug 01;8(8):5598-5605. doi: 10.1039/c7sc01364g. Epub 2017 May 30. PMID: 28970939; PMCID: PMC5618792.
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Chem Sci. 2017 Aug 01;8(8):5598-5605. doi: 10.1039/c7sc01364g. Epub 2017 May 30.

Dynamic multicolor protein labeling in living cells.

Chemical science

Chenge Li, Marie-Aude Plamont, Hanna L Sladitschek, Vanessa Rodrigues, Isabelle Aujard, Pierre Neveu, Thomas Le Saux, Ludovic Jullien, Arnaud Gautier

Affiliations

  1. École Normale Supérieure , PSL Research University , UPMC Univ Paris 06 , CNRS , Département de Chimie , PASTEUR , 24 rue Lhomond , 75005 Paris , France.
  2. Sorbonne Universités , UPMC Univ Paris 06 , ENS , CNRS , PASTEUR , 75005 Paris , France . Email: [email protected] ; Email: [email protected].
  3. Cell Biology and Biophysics Unit , European Molecular Biology Laboratory , Meyerhofstr. 1 , D-69117 Heidelberg , Germany.

PMID: 28970939 PMCID: PMC5618792 DOI: 10.1039/c7sc01364g

Abstract

Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST, hereafter called FAST) is a 14 kDa protein tag giving a bright green-yellow fluorescent complex upon interaction with the fluorogenic dye 4-hydroxy-3-methylbenzylidene rhodanine (HMBR). Here, we report a collection of fluorogens enabling tuning of the fluorescence color of FAST from green-yellow to orange and red. Beyond allowing the multicolor imaging of FAST-tagged proteins in live cells, these fluorogens enable dynamic color switching because of FAST's reversible labeling. This unprecedented behavior allows for selective detection of FAST-tagged proteins in cells expressing both green and red fluorescent species through two-color cross-correlation, opening up exciting prospects to overcome spectral crowding and push the frontiers of multiplexed imaging.

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