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Awata R, Sawai H, Imai K, et al. Morphological comparison and functional reconstitution of rat hepatic parenchymal cells on various matrices. J Gastroenterol Hepatol. 1998;13:S55-S61doi: 10.1111/jgh.1998.13.s1.55.
Awata, R., Sawai, H., Imai, K., Terada, K., Senoo, H., & Sugiyama, T. (1998). Morphological comparison and functional reconstitution of rat hepatic parenchymal cells on various matrices. Journal of gastroenterology and hepatology, 13S55-S61. https://doi.org/10.1111/jgh.1998.13.s1.55
Awata, Ryoichi, et al. "Morphological comparison and functional reconstitution of rat hepatic parenchymal cells on various matrices." Journal of gastroenterology and hepatology vol. 13 (1998): S55-S61. doi: https://doi.org/10.1111/jgh.1998.13.s1.55
Awata R, Sawai H, Imai K, Terada K, Senoo H, Sugiyama T. Morphological comparison and functional reconstitution of rat hepatic parenchymal cells on various matrices. J Gastroenterol Hepatol. 1998 Sep;13:S55-S61. doi: 10.1111/jgh.1998.13.s1.55. PMID: 28976700.
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J Gastroenterol Hepatol. 1998 Sep;13:S55-S61. doi: 10.1111/jgh.1998.13.s1.55.

Morphological comparison and functional reconstitution of rat hepatic parenchymal cells on various matrices.

Journal of gastroenterology and hepatology

Ryoichi Awata, Hiroshi Sawai, Katsuyuki Imai, Kunihiko Terada, Haruki Senoo, Toshihiro Sugiyama

Affiliations

  1. Akita Sumitomo Bakelite Co., Ltd.
  2. Akita University School of Medicine Department of Anatomy, Akita, Japan.
  3. Akita University School of Medicine Department of Biochemistry, Akita, Japan.

PMID: 28976700 DOI: 10.1111/jgh.1998.13.s1.55

Abstract

Four types of materials, type I collagen coat (Coat), acid-soluble type I collagen gel (Hardgel), pepsin-treated acid-soluble type I collagen gel (Softgel), and an extract of extracellular matrix of the murine Engelbreth-Holm-Swarm sarcoma (Matrigel), were used as matrices to culture rat hepatic parenchymal cells, and their morphological changes and adhesion were compared to the matrices by electron microscopic observations. Hepatic parenchymal cells cultured on Coat and Hardgel were extended and flattened, whereas cells cultured on Softgel and Matrigel assembled and formed aggregates. Such aggregates consisted of several hepatic parenchymal cells, with a recognizable bile duct-like alveolus on the inside. Morphologically, the aggregates were more spherical on Matrigel and oval shaped on Softgel. Microvilli of the cell surface were parallel to the matrix on Matrigel, but invaded into the gel on Softgel. Subsequently, investigation into how these morphological features affected the liver-specific functions, including secretion of albumin and induction of P450 by 3-methylcholanthrene, demonstrated that a high level of liver function was maintained in a long-term culture in hepatic parenchymal cells on Softgel. These results suggest that hepatic parenchymal cell interactions were stronger with Softgel than with Matrigel, and that Softgel appears to closely mimic the in vivo environment.

© 1998 The Official Publication of the Asian Pacific Association for the Study of the Liver and the Asian Pacific Association of Gastroenterology.

Keywords: collagen gel; hepatic parenchymal cell; hepatocyte; three-dimensional culture

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