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Kokura K, Nakadai T, Kishimoto T, et al. Gene expression in hepatomas. J Gastroenterol Hepatol. 1998;13:S132-S141doi: 10.1111/jgh.1998.13.s1.132.
Kokura, K., Nakadai, T., Kishimoto, T., Makino, Y., Muramatsu, M., & Tamura, T. A. (1998). Gene expression in hepatomas. Journal of gastroenterology and hepatology, 13S132-S141. https://doi.org/10.1111/jgh.1998.13.s1.132
Kokura, Kenji, et al. "Gene expression in hepatomas." Journal of gastroenterology and hepatology vol. 13 (1998): S132-S141. doi: https://doi.org/10.1111/jgh.1998.13.s1.132
Kokura K, Nakadai T, Kishimoto T, Makino Y, Muramatsu M, Tamura TA. Gene expression in hepatomas. J Gastroenterol Hepatol. 1998 Sep;13:S132-S141. doi: 10.1111/jgh.1998.13.s1.132. PMID: 28976702.
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J Gastroenterol Hepatol. 1998 Sep;13:S132-S141. doi: 10.1111/jgh.1998.13.s1.132.

Gene expression in hepatomas.

Journal of gastroenterology and hepatology

Kenji Kokura, Tomoyoshi Nakadai, Toshihiko Kishimoto, Yasutaka Makino, Masami Muramatsu, Taka-Aki Tamura

Affiliations

  1. Department of Biology, Faculty of Science, Chiba University, Chiba.
  2. Department of Biochemistry, Saitama Medical School, Saitama, Japan.

PMID: 28976702 DOI: 10.1111/jgh.1998.13.s1.132

Abstract

Gene expression changes in accordance with cell growth, differentiation and carcinogenesis. To elucidate the molecular mechanisms for hepatocarcinogenesis as well as maintenance of normal hepatocytes, it is important to identify the genes that have altered expression with carcinogenesis. We established a new and efficient cDNA subtraction method via two cDNA populations. By using this method along with rat hepatomas made by the Soh-Farber protocol, we identified a number of genes, some of which are activated in hepatocellular carcinoma (HCC). These genes include ones which code for a transcription factor and a metabolic enzyme. One particular gene can be used as a tumour marker. Our method is beneficial for the isolation of a wide range of HCC-related genes in rats which, in turn, enables easy identification of their human counterparts. In this review, we describe details of our method and the isolated genes. We also briefly describe transcription factors in the liver.

© 1998 The Official Publication of the Asian Pacific Association for the Study of the Liver and the Asian Pacific Association of Gastroenterology.

Keywords: Solt-Farber protocol; cDNA subtraction; hepatocarcinogenesis; hepatocellular carcinoma; transcription factor

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