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Immune Netw. 2017 Aug;17(4):261-268. doi: 10.4110/in.2017.17.4.261. Epub 2017 Aug 11.

Localization of Serum Amyloid A3 in the Mouse Ovary.

Immune network

Hyeongjwa Choi, Rosa Mistica C Ignacio, Eun-Sook Lee, Katherine F Roby, Paul F Terranova, Deok-Soo Son

Affiliations

  1. Department of Biochemistry and Cancer Biology, Meharry Medical College, Nashville, TN 37208, USA.
  2. Department of Pharmaceutical Sciences, College of Pharmacy, Florida A&M University, Tallahassee, FL 32301, USA.
  3. Center for Reproductive Sciences, University of Kansas Medical Center, Kansas City, KS 66160, USA.
  4. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
  5. Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
  6. Department of Obstetrics and Gynecology, University of Kansas Medical Center, Kansas City, KS 66160, USA.

PMID: 28860955 PMCID: PMC5577303 DOI: 10.4110/in.2017.17.4.261

Abstract

Tumor necrosis factor-α (TNF-α) induces serum amyloid A (SAA) 3 among acute-phase proteins in mouse granulosa cells by activating NF-κB signaling via p55 TNF-α receptor type 1. However, the localization of SAA3 within the ovary is unknown. Here we investigated ovarian localization of SAA3 in a mouse ovulation model and in response to IL-1β, a proinflammatory mediator. For the ovulation model, equine chorionic gonadotropin (eCG; 2.5 IU) was administered to mice subcutaneously (sc) to stimulate follicular development on day 25 of age and then 50 h after eCG, human chorionic gonadotropin (hCG; 2.5 IU) was administered sc to induce ovulation. The mouse ovulation model was characterized by the localization of CYP19 mRNA expression to granulosa layers of larger follicles. SAA3 mRNA, determined by

Keywords: Ovary; Ovulation; Serum amyloid A

Conflict of interest statement

Conflict of Interest: The authors declare no potential conflicts of interest.

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