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Nair R, Manikkath J, Hegde AR, et al. Liposome-encapsulated diacyl glycerol and inositol triphosphate-induced delayed oocyte activation and poor development of parthenotes. J Turk Ger Gynecol Assoc. 2017;18(3):102-109doi: 10.4274/jtgga.2017.0014.
Nair, R., Manikkath, J., Hegde, A. R., Mutalik, S., Kalthur, G., & Adiga, S. K. (2017). Liposome-encapsulated diacyl glycerol and inositol triphosphate-induced delayed oocyte activation and poor development of parthenotes. Journal of the Turkish German Gynecological Association, 18(3), 102-109. https://doi.org/10.4274/jtgga.2017.0014
Nair, Ramya, et al. "Liposome-encapsulated diacyl glycerol and inositol triphosphate-induced delayed oocyte activation and poor development of parthenotes." Journal of the Turkish German Gynecological Association vol. 18,3 (2017): 102-109. doi: https://doi.org/10.4274/jtgga.2017.0014
Nair R, Manikkath J, Hegde AR, Mutalik S, Kalthur G, Adiga SK. Liposome-encapsulated diacyl glycerol and inositol triphosphate-induced delayed oocyte activation and poor development of parthenotes. J Turk Ger Gynecol Assoc. 2017 Sep 01;18(3):102-109. doi: 10.4274/jtgga.2017.0014. PMID: 28890423; PMCID: PMC5590204.
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J Turk Ger Gynecol Assoc. 2017 Sep 01;18(3):102-109. doi: 10.4274/jtgga.2017.0014.

Liposome-encapsulated diacyl glycerol and inositol triphosphate-induced delayed oocyte activation and poor development of parthenotes.

Journal of the Turkish German Gynecological Association

Ramya Nair, Jyothsna Manikkath, Aswathi R Hegde, Srinivas Mutalik, Guruprasad Kalthur, Satish Kumar Adiga

Affiliations

  1. Department of Clinical Embryology, Central Research Lab, Kasturba Medical College, Manipal University, Manipal, India.
  2. Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India.

PMID: 28890423 PMCID: PMC5590204 DOI: 10.4274/jtgga.2017.0014

Abstract

OBJECTIVE: To explore the ability of diacyl glycerol (DAG) and inositol triphosphate (IP3), two major secondary messengers in the calcium signaling pathway, in activating oocytes.

MATERIAL AND METHODS: Oocyte cumulus complex obtained from superovulated Swiss albino mice were incubated in M16 medium with liposome-encapsulated 1,2-Dipalmitoyl-sn-glycerol (LEDAG) and/or IP3 for 3 h. Strontium chloride was used as positive control. The activation potential, ploidy status, and blastocyst rate was calculated.

RESULTS: Both DAG and IP3, individually, induced activation in ~98% of oocytes, which was significantly higher (p<0.01) than activation induced by strontium chloride (60%). Delayed pronucleus formation and a higher percentage of diploid parthenotes was observed in oocytes activated with LEDAG and/or IP3. However, these embryos failed to progress beyond the 6-8-cell stage. Only when the medium was supplemented with LEDAG (5 μg/mL) and IP3 (10 μg/mL) could activated oocytes progress till the blastocyst stage (5.26%), which was lower than the blastocyst rate in the positive controls (13.91%).

CONCLUSION: The results of the present study indicate that DAG and IP3 can induce delayed oocyte activation and poor development of parthenotes in vitro.

Keywords: Oocyte activation; diacyl glycerol; inositol triphosphate; liposomes embryo development.

References

  1. Mol Reprod Dev. 1991 Nov;30(3):214-9 - PubMed
  2. J Physiol. 1994 Nov 1;480 ( Pt 3):465-74 - PubMed
  3. Molecules. 2014 Apr 15;19(4):4708-44 - PubMed
  4. Dev Biol. 2000 Sep 1;225(1):1-12 - PubMed
  5. J Assist Reprod Genet. 1996 May;13(5):435-46 - PubMed
  6. Science. 2000 Mar 3;287(5458):1647-51 - PubMed
  7. Cancer Res. 1990 Mar 1;50(5):1431-9 - PubMed
  8. Development. 1991 Mar;111(3):763-9 - PubMed
  9. Int Rev Cytol. 1994;152:183-222 - PubMed
  10. Curr Top Dev Biol. 1995;30:21-62 - PubMed
  11. Science. 1991 Apr 19;252(5004):443-6 - PubMed
  12. Theriogenology. 1993 Aug;40(2):257-66 - PubMed
  13. Cell Rep. 2013 Dec 12;5(5):1375-86 - PubMed
  14. Mol Pain. 2008 Oct 01;4:42 - PubMed
  15. Proc Natl Acad Sci U S A. 2014 Nov 18;111(46):E4972-80 - PubMed
  16. Biol Reprod. 1998 Jan;58(1):94-102 - PubMed
  17. J Gen Physiol. 1996 Sep;108(3):157-75 - PubMed
  18. Cell Calcium. 1994 Apr;15(4):331-9 - PubMed
  19. J Membr Biol. 1982;67(3):165-82 - PubMed
  20. Nature. 1985 Aug 8-14;316(6028):541-2 - PubMed
  21. J Neurosci. 2009 Aug 12;29(32):10000-9 - PubMed
  22. Biochim Biophys Acta. 2009 Jun;1793(6):933-40 - PubMed
  23. Nat Commun. 2016 Jan 04;7:10253 - PubMed
  24. Mol Reprod Dev. 1996 Mar;43(3):313-22 - PubMed
  25. Fertil Steril. 2010 Feb;93(3):959-66 - PubMed
  26. J Exp Zool. 1983 May;226(2):311-4 - PubMed
  27. J Biol Chem. 1998 Feb 13;273(7):4052-8 - PubMed
  28. Dev Biol. 1993 Sep;159(1):122-30 - PubMed
  29. Science. 1992 Oct 23;258(5082):607-14 - PubMed
  30. Dev Biol. 1992 Jan;149(1):80-9 - PubMed
  31. Nature. 1991 Jun 27;351(6329):751-4 - PubMed
  32. J Anim Sci. 2004;82 E-Suppl:E14-23 - PubMed
  33. Zygote. 1993 Aug;1(3):243-56 - PubMed
  34. Mol Reprod Dev. 1997 Apr;46(4):587-601 - PubMed
  35. Dev Biol. 1996 Sep 15;178(2):229-37 - PubMed

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