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Song WS, Lee JK, Park SH, et al. Comparison of periodontitis-associated oral biofilm formation under dynamic and static conditions. J Periodontal Implant Sci. 2017;47(4):219-230doi: 10.5051/jpis.2017.47.4.219.
Song, W. S., Lee, J. K., Park, S. H., Um, H. S., Lee, S. Y., & Chang, B. S. (2017). Comparison of periodontitis-associated oral biofilm formation under dynamic and static conditions. Journal of periodontal & implant science, 47(4), 219-230. https://doi.org/10.5051/jpis.2017.47.4.219
Song, Won Sub, et al. "Comparison of periodontitis-associated oral biofilm formation under dynamic and static conditions." Journal of periodontal & implant science vol. 47,4 (2017): 219-230. doi: https://doi.org/10.5051/jpis.2017.47.4.219
Song WS, Lee JK, Park SH, Um HS, Lee SY, Chang BS. Comparison of periodontitis-associated oral biofilm formation under dynamic and static conditions. J Periodontal Implant Sci. 2017 Aug;47(4):219-230. doi: 10.5051/jpis.2017.47.4.219. Epub 2017 Aug 16. PMID: 28861286; PMCID: PMC5577440.
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J Periodontal Implant Sci. 2017 Aug;47(4):219-230. doi: 10.5051/jpis.2017.47.4.219. Epub 2017 Aug 16.

Comparison of periodontitis-associated oral biofilm formation under dynamic and static conditions.

Journal of periodontal & implant science

Won Sub Song, Jae-Kwan Lee, Se Hwan Park, Heung-Sik Um, Si Young Lee, Beom-Seok Chang

Affiliations

  1. Department of Periodontology and Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry, Gangneung, Korea.
  2. Department of Microbiology and Immunology and Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry, Gangneung, Korea.

PMID: 28861286 PMCID: PMC5577440 DOI: 10.5051/jpis.2017.47.4.219

Abstract

PURPOSE: The purpose of this study was to compare the characteristics of single- and dual-species

METHODS: Hydroxyapatite (HA) disks, 12.7 mm in diameter and 3 mm thick, were coated with processed saliva for 4 hours. The disks were divided into a static method group and a dynamic method group. The disks treated with a static method were cultured in 12-well plates, and the disks in the dynamic method group were cultured in a Center for Disease Control and Prevention (CDC) biofilm reactor for 72 hours. In the single- and dual-species biofilms,

RESULTS: Compared with the biofilms made using the static method, the biofilms made using the dynamic method had significantly lower amounts of adhering and looser bacterial accumulation in SEM and CLSM images. The proportion of

CONCLUSIONS: When used to reproduce periodontal biofilms composed of

Keywords: Bacterial adhesion; Biofilms; Chlorhexidine; Electron microscope tomography; Periodontitis

Conflict of interest statement

Conflict of Interest: No potential conflict of interest relevant to this article was reported.

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