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Gillespie EL, Pauley AG, Haffner ML, et al. Fourteen polymorphic microsatellite markers for a widespread limestone endemic, . Appl Plant Sci. 2017;5(8)doi: 10.3732/apps.1700031.
Gillespie, E. L., Pauley, A. G., Haffner, M. L., Hay, N. M., Estep, M. C., & Murrell, Z. E. (2017). Fourteen polymorphic microsatellite markers for a widespread limestone endemic, . Applications in plant sciences, 5(8), . https://doi.org/10.3732/apps.1700031
Gillespie, Emily L, et al. "Fourteen polymorphic microsatellite markers for a widespread limestone endemic, ." Applications in plant sciences vol. 5,8 (2017). doi: https://doi.org/10.3732/apps.1700031
Gillespie EL, Pauley AG, Haffner ML, Hay NM, Estep MC, Murrell ZE. Fourteen polymorphic microsatellite markers for a widespread limestone endemic, . Appl Plant Sci. 2017 Aug 21;5(8). doi: 10.3732/apps.1700031. eCollection 2017 Aug. PMID: 28924512; PMCID: PMC5584816.
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Appl Plant Sci. 2017 Aug 21;5(8). doi: 10.3732/apps.1700031. eCollection 2017 Aug.

Fourteen polymorphic microsatellite markers for a widespread limestone endemic, .

Applications in plant sciences

Emily L Gillespie, Annabella G Pauley, Megan L Haffner, Nikolai M Hay, Matt C Estep, Zack E Murrell

Affiliations

  1. Department of Biological Sciences, Marshall University, One John Marshall Drive, Huntington, West Virginia 25755 USA.
  2. Department of Biology, Appalachian State University, 572 Rivers Street, Boone, North Carolina 28607 USA.

PMID: 28924512 PMCID: PMC5584816 DOI: 10.3732/apps.1700031

Abstract

PREMISE OF THE STUDY: Microsatellite primers were developed for a widespread limestone endemic sedge,

METHODS AND RESULTS: Forty-eight primer pairs were designed from Illumina sequence data and screened for suitability. Fourteen of these primer pairs were polymorphic and generated one to seven alleles per locus. Cross-species amplifications were conducted for all four members of

CONCLUSIONS: These primer pairs can be used to assess the genetic diversity and population structure in future studies of

Keywords: Carex eburnea; Carex mckittrickensis; Carex sect. Albae; Cyperaceae; genetic diversity; limestone endemic

References

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