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Sperber J, Nyberg A, Lipcsey M, et al. Protective ventilation reduces Pseudomonas aeruginosa growth in lung tissue in a porcine pneumonia model. Intensive Care Med Exp. 2017;5(1):40doi: 10.1186/s40635-017-0152-3.
Sperber, J., Nyberg, A., Lipcsey, M., Melhus, �. �., Larsson, A., Sjölin, J., & Castegren, M. (2017). Protective ventilation reduces Pseudomonas aeruginosa growth in lung tissue in a porcine pneumonia model. Intensive care medicine experimental, 5(1), 40. https://doi.org/10.1186/s40635-017-0152-3
Sperber, Jesper, et al. "Protective ventilation reduces Pseudomonas aeruginosa growth in lung tissue in a porcine pneumonia model." Intensive care medicine experimental vol. 5,1 (2017): 40. doi: https://doi.org/10.1186/s40635-017-0152-3
Sperber J, Nyberg A, Lipcsey M, Melhus Å, Larsson A, Sjölin J, Castegren M. Protective ventilation reduces Pseudomonas aeruginosa growth in lung tissue in a porcine pneumonia model. Intensive Care Med Exp. 2017 Aug 31;5(1):40. doi: 10.1186/s40635-017-0152-3. PMID: 28861863; PMCID: PMC5578946.
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Intensive Care Med Exp. 2017 Aug 31;5(1):40. doi: 10.1186/s40635-017-0152-3.

Protective ventilation reduces Pseudomonas aeruginosa growth in lung tissue in a porcine pneumonia model.

Intensive care medicine experimental

Jesper Sperber, Axel Nyberg, Miklos Lipcsey, Åsa Melhus, Anders Larsson, Jan Sjölin, Markus Castegren

Affiliations

  1. Centre for Clinical Research Sörmland, Uppsala University, Uppsala, Sweden. [email protected].
  2. Centre for Clinical Research Sörmland, Department of Anesthesiology & Intensive Care Mälarsjukhuset, SE-631 88, Eskilstuna, Sweden. [email protected].
  3. Centre for Clinical Research Sörmland, Uppsala University, Uppsala, Sweden.
  4. Centre for Clinical Research Sörmland, Department of Anesthesiology & Intensive Care Mälarsjukhuset, SE-631 88, Eskilstuna, Sweden.
  5. Hedenstierna laboratory, Department of Surgical Sciences, Anesthesiology and Intensive Care, Uppsala University, Uppsala, Sweden.
  6. Department of Medical Sciences, Section of Clinical Microbiology, Uppsala University, Uppsala, Sweden.
  7. Department of Medical Sciences, Biochemical structure and function, Uppsala University, Uppsala, Sweden.
  8. Department of Medical Sciences, Infectious Diseases, Uppsala University, Uppsala, Sweden.
  9. Perioperative Medicine and Intensive Care, Karolinska University Hospital and CLINTEC, Karolinska Institute, Stockholm, Sweden.

PMID: 28861863 PMCID: PMC5578946 DOI: 10.1186/s40635-017-0152-3

Abstract

BACKGROUND: Mechanical ventilation with positive end expiratory pressure and low tidal volume, i.e. protective ventilation, is recommended in patients with acute respiratory distress syndrome. However, the effect of protective ventilation on bacterial growth during early pneumonia in non-injured lungs is not extensively studied. The main objectives were to compare two different ventilator settings on Pseudomonas aeruginosa growth in lung tissue and the development of lung injury.

METHODS: A porcine model of severe pneumonia was used. The protective group (n = 10) had an end expiratory pressure of 10 cm H

RESULTS: The protective group displayed lower numbers of Pseudomonas aeruginosa (p < 0.05) in the lung tissue, and a lower wet-to-dry ratio (p < 0.01) than the control group. The control group deteriorated in arterial oxygen tension/inspired oxygen fraction, whereas the protective group was unchanged (p < 0.01).

CONCLUSIONS: In early phase pneumonia, protective ventilation with lower tidal volume and higher end expiratory pressure has the potential to reduce the pulmonary bacterial burden and the development of lung injury.

Keywords: Bacterial infections; Critical care; Models, animal; Protective ventilation; Ventilators, mechanical

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