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Zeltins A, Turks M, Skrastina D, et al. Synthesis and Immunological Evaluation of Virus-Like Particle-Milbemycin A₃/A₄ Conjugates. Antibiotics (Basel). 2017;6(3)doi: 10.3390/antibiotics6030018.
Zeltins, A., Turks, M., Skrastina, D., Lugiņina, J., Kalnciema, I., Balke, I., Bizdēna, �. �., & Skrivelis, V. (2017). Synthesis and Immunological Evaluation of Virus-Like Particle-Milbemycin A₃/A₄ Conjugates. Antibiotics (Basel, Switzerland), 6(3), . https://doi.org/10.3390/antibiotics6030018
Zeltins, Andris, et al. "Synthesis and Immunological Evaluation of Virus-Like Particle-Milbemycin A₃/A₄ Conjugates." Antibiotics (Basel, Switzerland) vol. 6,3 (2017). doi: https://doi.org/10.3390/antibiotics6030018
Zeltins A, Turks M, Skrastina D, Lugiņina J, Kalnciema I, Balke I, Bizdēna Ē, Skrivelis V. Synthesis and Immunological Evaluation of Virus-Like Particle-Milbemycin A₃/A₄ Conjugates. Antibiotics (Basel). 2017 Sep 11;6(3). doi: 10.3390/antibiotics6030018. PMID: 28892001; PMCID: PMC5617982.
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Antibiotics (Basel). 2017 Sep 11;6(3). doi: 10.3390/antibiotics6030018.

Synthesis and Immunological Evaluation of Virus-Like Particle-Milbemycin A₃/A₄ Conjugates.

Antibiotics (Basel, Switzerland)

Andris Zeltins, Māris Turks, Dace Skrastina, Jevgeņija Lugiņina, Ieva Kalnciema, Ina Balke, Ērika Bizdēna, Vitalijs Skrivelis

Affiliations

  1. Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia. [email protected].
  2. Institute of Technology of Organic Chemistry, Riga Technical University, P. Valdena Str. 3, LV-1048 Riga, Latvia. [email protected].
  3. Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia. [email protected].
  4. Institute of Technology of Organic Chemistry, Riga Technical University, P. Valdena Str. 3, LV-1048 Riga, Latvia. [email protected].
  5. Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia. [email protected].
  6. Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia.
  7. Institute of Technology of Organic Chemistry, Riga Technical University, P. Valdena Str. 3, LV-1048 Riga, Latvia. [email protected].
  8. PharmIdea Ltd., Rupnicu 4, Olaine, Riga District, LV-2114 Olaine, Latvia. [email protected].

PMID: 28892001 PMCID: PMC5617982 DOI: 10.3390/antibiotics6030018

Abstract

Milbemycins are macrolide antibiotics with a broad spectrum of nematocidal, insecticidal, and acaricidal activity. To obtain milbemycin A₃/A₄ derivatives suitable for chemical conjugation to protein carriers (milbemycin haptens), succinate linker and a novel 17-atom-long linker containing a terminal carboxylic acid group were attached to the milbemycin core in a protecting group-free synthesis. The obtained milbemycin A₃/A₄ derivatives were coupled to Potato virus Y-like nanoparticles by the activated ester method. The reaction products were characterized and used in mice immunization experiments. It was found that the mice developed weak specific immune responses toward all tested milbemycin haptens.

Keywords: ELISA; hydrophobic hapten; macrocyclic lactone; plant virus

Conflict of interest statement

The authors declare no conflict of interest. The funder of the study had no role in study design, data analysis and interpretation, or writing of the article.

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