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Miki A, Narushima M, Okitsu T, et al. Maintenance of Mouse, Rat, and Pig Pancreatic Islet Functions by Coculture with Human Islet-Derived Fibroblasts. Cell Transplant. 2006;15(4):325-334doi: 10.3727/000000006783981882.
Miki, A., Narushima, M., Okitsu, T., Takeno, Y., Soto-Gutierrez, A., Rivas-Carrillo, J. D., Navarro-Alvarez, N., Chen, Y., Tanaka, K., Noguchi, H., Matsumoto, S., Kohara, M., Lakey, J. R. T., Kobayashi, E., Tanaka, N., & Kobayashi, N. (2006). Maintenance of Mouse, Rat, and Pig Pancreatic Islet Functions by Coculture with Human Islet-Derived Fibroblasts. Cell transplantation, 15(4), 325-334. https://doi.org/10.3727/000000006783981882
Miki, Atsushi, et al. "Maintenance of Mouse, Rat, and Pig Pancreatic Islet Functions by Coculture with Human Islet-Derived Fibroblasts." Cell transplantation vol. 15,4 (2006): 325-334. doi: https://doi.org/10.3727/000000006783981882
Miki A, Narushima M, Okitsu T, Takeno Y, Soto-Gutierrez A, Rivas-Carrillo JD, Navarro-Alvarez N, Chen Y, Tanaka K, Noguchi H, Matsumoto S, Kohara M, Lakey JRT, Kobayashi E, Tanaka N, Kobayashi N. Maintenance of Mouse, Rat, and Pig Pancreatic Islet Functions by Coculture with Human Islet-Derived Fibroblasts. Cell Transplant. 2006 Apr;15(4):325-334. doi: 10.3727/000000006783981882. PMID: 28863744.
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Cell Transplant. 2006 Apr;15(4):325-334. doi: 10.3727/000000006783981882.

Maintenance of Mouse, Rat, and Pig Pancreatic Islet Functions by Coculture with Human Islet-Derived Fibroblasts.

Cell transplantation

Atsushi Miki, Michiki Narushima, Teru Okitsu, Yuichi Takeno, Alejandro Soto-Gutierrez, Jorge David Rivas-Carrillo, Nalú Navarro-Alvarez, Yong Chen, Kimiaki Tanaka, Hirofumi Noguchi, Shinichi Matsumoto, Michinori Kohara, Jonathan R T Lakey, Eiji Kobayashi, Noriaki Tanaka, Naoya Kobayashi

Affiliations

  1. Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
  2. Department of Transplant Surgery, Kyoto University Hospital, 54 Seigoin-Kawaracho, Sakyo-ku, Kyoto 606-8507, Japan.
  3. Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi 329-0498, Japan.
  4. Department of Microbiology and Cell Biology, The Tokyo Metropolitan Institute of Medical Science, Honkomagome, Bunkyo-ku, Tokyo 113-8613 Japan.
  5. Human Pancreatic Islet Transplant Program, University of Alberta, Alberta T2N 4N1, Canada.

PMID: 28863744 DOI: 10.3727/000000006783981882

Abstract

Development of an efficient preculture system of islets is ideal. Toward that goal, we constructed a human pancreatic islet-derived fibroblast cell line MNNK-1 for a source as a coculture system for freshly isolated islets to maintain islet functions. Human pancreatic islet cells were nucleofected with a plasmid vector pYK-1 expressing simian virus 40 large T antigen gene (SV40T) and hygromycin resistance gene (HygroR). One of the transduced cell lines, MNNK-1, was established and served as a feeder cell in the coculture for freshly isolated mouse, rat, and pig islets. Morphology, viability, and glucose-responding insulin secretion were analyzed in the coculture system. MNNK-1 cells were morphologically spindle shaped and were negative for pancreatic endocrine markers. MNNK-1 cells were positive for α-smooth muscle actin and collagen type I and produced fibroblast growth factor. Coculture of the mouse, rat, and pig islets with MNNK-1 cells maintained their viability and insulin secretion with glucose responsiveness. A human pancreatic islet-derived fibroblast cell line MNNK-1 was established. MNNK-1 cells were a useful means for maintaining morphology and insulin secretion of islets in the coculture system.

Keywords: Coculture; Islets; Simian virus 40 large T antigen

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