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N Zekri AR, Salama H, Medhat E, et al. Potential Diagnostic and Prognostic Value of Lymphocytic Mitochondrial DNA Deletion in Relation to Folic Acid Status in HCV-Related Hepatocellular Carcinoma. Asian Pac J Cancer Prev. 2017;18(9):2451-2457doi: 10.22034/APJCP.2017.18.9.2451.
N Zekri, A. R., Salama, H., Medhat, E., Hamdy, S., Hassan, Z. K., Bakr, Y. M., Youssef, A. S. -. D., Saleh, D., Saeed, R., & Omran, D. (2017). Potential Diagnostic and Prognostic Value of Lymphocytic Mitochondrial DNA Deletion in Relation to Folic Acid Status in HCV-Related Hepatocellular Carcinoma. Asian Pacific journal of cancer prevention : APJCP, 18(9), 2451-2457. https://doi.org/10.22034/APJCP.2017.18.9.2451
N Zekri, Abdel Rahman, et al. "Potential Diagnostic and Prognostic Value of Lymphocytic Mitochondrial DNA Deletion in Relation to Folic Acid Status in HCV-Related Hepatocellular Carcinoma." Asian Pacific journal of cancer prevention : APJCP vol. 18,9 (2017): 2451-2457. doi: https://doi.org/10.22034/APJCP.2017.18.9.2451
N Zekri AR, Salama H, Medhat E, Hamdy S, Hassan ZK, Bakr YM, Youssef AS-D, Saleh D, Saeed R, Omran D. Potential Diagnostic and Prognostic Value of Lymphocytic Mitochondrial DNA Deletion in Relation to Folic Acid Status in HCV-Related Hepatocellular Carcinoma. Asian Pac J Cancer Prev. 2017 Sep 27;18(9):2451-2457. doi: 10.22034/APJCP.2017.18.9.2451. PMID: 28952275; PMCID: PMC5720650.
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Asian Pac J Cancer Prev. 2017 Sep 27;18(9):2451-2457. doi: 10.22034/APJCP.2017.18.9.2451.

Potential Diagnostic and Prognostic Value of Lymphocytic Mitochondrial DNA Deletion in Relation to Folic Acid Status in HCV-Related Hepatocellular Carcinoma.

Asian Pacific journal of cancer prevention : APJCP

Abdel Rahman N Zekri, Hosny Salama, Eman Medhat, Sherif Hamdy, Zeinab K Hassan, Yasser Mabrouk Bakr, Amira Salah El - Din Youssef, Doaa Saleh, Ramy Saeed, Dalia Omran

Affiliations

  1. Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt. Email: [email protected]

PMID: 28952275 PMCID: PMC5720650 DOI: 10.22034/APJCP.2017.18.9.2451

Abstract

Objective: We assessed the possibility of using mitochondrial (mt) DNA deletion as a molecular biomarker for disease progression in HCV-related hepatocellular carcinoma (HCC) and to identify its association with folic acid status. Methods: Serum folic acid and lymphocytic mtDNA deletions were assessed in 90 patients; 50 with HCC, 20 with liver cirrhosis (LC), and 20 with chronic hepatitis C (CHC) compared to 10 healthy control subjects. The diagnostic accuracy of mtDNA deletions frequency was evaluated using receiver-operating characteristic (ROC) curve analysis Survival analysis was performed using the Kaplan-Meier method. Differences in the survival rates were compared using log-rank test. Result: Our data revealed a significant elevation of mtDNA deletions frequency in the HCC group compared to the other groups (P-value <0.01). Also, our data showed a significant correlation between folate deficiency and high frequency of mtDNA deletions in patients with HCV-related HCC when compared to the other groups (r= -0.094 and P-value <0.05). Moreover, the size of the hepatic focal lesion in the HCC patients was positively correlated with mtDNA deletions (r= 0.09 and P-value <0.01). The median survival time for the HCC patients with high frequency of mtDNA deletions (ΔCt ≥3.9; 5.7+ 0.6 months) was significantly shorter than those with low mtDNA deletions frequency (ΔCt < 3.9; 11.9+ 0.04 months, P-value <0.01). Conclusion: Our data provided an evidence that lymphocytic mtDNA deletion could be used as non-invasive biomarker for disease progression and patients’ survival in HCV-related HCC. Also, our findings implied a causal relationship between the folate deficiency and the high mtDNA deletions frequency among Egyptian patients with HCV related HCC.

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Keywords: HCC; Egyptian patients; mitochondrial DNA deletions; serum folic acid; diagnosis; prognosis

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