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Cai J, Xin H, Cheng L, et al. Preparative separation of the polar part from the rhizomes of Anemarrhena asphodeloides using a hydrophilic C18 stationary phase. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1063:149-155doi: 10.1016/j.jchromb.2017.08.034.
Cai, J., Xin, H., Cheng, L., Fu, Y., Jiang, D., Feng, J., Fu, Q., Jin, Y., & Liang, X. (2017). Preparative separation of the polar part from the rhizomes of Anemarrhena asphodeloides using a hydrophilic C18 stationary phase. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1063149-155. https://doi.org/10.1016/j.jchromb.2017.08.034
Cai, Jianfeng, et al. "Preparative separation of the polar part from the rhizomes of Anemarrhena asphodeloides using a hydrophilic C18 stationary phase." Journal of chromatography. B, Analytical technologies in the biomedical and life sciences vol. 1063 (2017): 149-155. doi: https://doi.org/10.1016/j.jchromb.2017.08.034
Cai J, Xin H, Cheng L, Fu Y, Jiang D, Feng J, Fu Q, Jin Y, Liang X. Preparative separation of the polar part from the rhizomes of Anemarrhena asphodeloides using a hydrophilic C18 stationary phase. J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 15;1063:149-155. doi: 10.1016/j.jchromb.2017.08.034. Epub 2017 Aug 31. PMID: 28865331.
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J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 15;1063:149-155. doi: 10.1016/j.jchromb.2017.08.034. Epub 2017 Aug 31.

Preparative separation of the polar part from the rhizomes of Anemarrhena asphodeloides using a hydrophilic C18 stationary phase.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Jianfeng Cai, Huaxia Xin, Lingping Cheng, YanHui Fu, Dasen Jiang, Jiatao Feng, Qing Fu, Yu Jin, Xinmiao Liang

Affiliations

  1. Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
  2. Hai Nan Normal University, 99 South Longkun Road, Haikou, 571127, PR China.
  3. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical, Physics, Chinese Academy of Science, Dalian 116203, PR China.
  4. Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: [email protected].
  5. Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China; Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical, Physics, Chinese Academy of Science, Dalian 116203, PR China.

PMID: 28865331 DOI: 10.1016/j.jchromb.2017.08.034

Abstract

The goal of this study was to develop a method that utilized a hydrophilic C18 stationary phase in the preparative high performance liquid chromatography to isolate the polar part from the rhizomes of Anemarrhena asphodeloides. The results showed that an initial mobile phase of pure water for the separation could greatly increase the retention and solubility of the polar compounds at the preparative scale. Introducing polar groups on the surface of the hydrophilic C18 column together with the use of optimized mobile phase compositions improved the column separation selectivity for polar compounds. Eleven previously undescribed compounds in Anemarrhena asphodeloides were obtained, indicating that the method developed in this study would facilitate the purification and separation of the polar part of traditional Chinese medicines.

Copyright © 2017 Elsevier B.V. All rights reserved.

Keywords: Anemarrhena asphodeloides; Hydrophilic C18 stationary phase; Polar part; Preparative high performance liquid chromatography

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