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Wanyonyi SS, Kumar A, Du Preez R, et al. Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix. Biochem Biophys Rep. 2017;12:120-128doi: 10.1016/j.bbrep.2017.08.013.
Wanyonyi, S. S., Kumar, A., Du Preez, R., Lefevre, C., & Nicholas, K. R. (2017). Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix. Biochemistry and biophysics reports, 12120-128. https://doi.org/10.1016/j.bbrep.2017.08.013
Wanyonyi, Stephen S, et al. "Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix." Biochemistry and biophysics reports vol. 12 (2017): 120-128. doi: https://doi.org/10.1016/j.bbrep.2017.08.013
Wanyonyi SS, Kumar A, Du Preez R, Lefevre C, Nicholas KR. Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix. Biochem Biophys Rep. 2017 Sep 05;12:120-128. doi: 10.1016/j.bbrep.2017.08.013. eCollection 2017 Dec. PMID: 28955800; PMCID: PMC5613237.
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Biochem Biophys Rep. 2017 Sep 05;12:120-128. doi: 10.1016/j.bbrep.2017.08.013. eCollection 2017 Dec.

Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix.

Biochemistry and biophysics reports

Stephen S Wanyonyi, Amit Kumar, Ryan Du Preez, Christophe Lefevre, Kevin R Nicholas

Affiliations

  1. Institute for Agriculture and the Environment, University of Southern Queensland, West St., Toowoomba, QLD, Australia.
  2. School of Medicine, Deakin University, VIC, Australia.
  3. Walter and Eliza Hall Institute of Medical Research, VIC, Australia.
  4. Anatomy & Developmental Biology, Monash University, VIC, Australia.

PMID: 28955800 PMCID: PMC5613237 DOI: 10.1016/j.bbrep.2017.08.013

Abstract

BACKGROUND: The unique lactation strategy of the tammar wallaby

METHODS: WallMEC from MID lactation mammary glands were cultured on ECM from MID or LATE lactation and treated for 5 days with 1 μg/ml cortisol, 1 μg/ml insulin, 0.2 µg/ml prolactin, 650 pg/ml triodothyronine and 1 pg/ml estradiol to induce lactation. WallMEC RNA from triplicate ECM treatments was used to perform RNAseq.

RESULTS: ECM from MID and LATE lactation differentially regulated key genes in sugar and lipid metabolism. Seven pathways including galactose metabolism, lysosome, cell adhesion molecules (CAM), p53 signaling, the complement and coagulation and Nod-like receptor signaling pathways were only significantly responsive to ECM in the presence of hormones. The raw RNA-seq data for this project are available on the NCBI Gene Expression Omnibus (GEO) browser (accession number GSE81210).

CONCLUSIONS: A potential role of ECM in regulation of the caloric content of milk, among other functions including apoptosis, cell proliferation and differentiation has been identified.

GENERAL SIGNIFICANCE: This study has used a non-eutherian lactation model to demonstrate the synergy between ECM and hormones in the local regulation of mammary function.

Keywords: Milk lipid composition

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