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Pervin M, Unno K, Nakagawa A, et al. Blood brain barrier permeability of (-)-epigallocatechin gallate, its proliferation-enhancing activity of human neuroblastoma SH-SY5Y cells, and its preventive effect on age-related cognitive dysfunction in mice. Biochem Biophys Rep. 2017;9:180-186doi: 10.1016/j.bbrep.2016.12.012.
Pervin, M., Unno, K., Nakagawa, A., Takahashi, Y., Iguchi, K., Yamamoto, H., Hoshino, M., Hara, A., Takagaki, A., Nanjo, F., Minami, A., Imai, S., & Nakamura, Y. (2017). Blood brain barrier permeability of (-)-epigallocatechin gallate, its proliferation-enhancing activity of human neuroblastoma SH-SY5Y cells, and its preventive effect on age-related cognitive dysfunction in mice. Biochemistry and biophysics reports, 9180-186. https://doi.org/10.1016/j.bbrep.2016.12.012
Pervin, Monira, et al. "Blood brain barrier permeability of (-)-epigallocatechin gallate, its proliferation-enhancing activity of human neuroblastoma SH-SY5Y cells, and its preventive effect on age-related cognitive dysfunction in mice." Biochemistry and biophysics reports vol. 9 (2017): 180-186. doi: https://doi.org/10.1016/j.bbrep.2016.12.012
Pervin M, Unno K, Nakagawa A, Takahashi Y, Iguchi K, Yamamoto H, Hoshino M, Hara A, Takagaki A, Nanjo F, Minami A, Imai S, Nakamura Y. Blood brain barrier permeability of (-)-epigallocatechin gallate, its proliferation-enhancing activity of human neuroblastoma SH-SY5Y cells, and its preventive effect on age-related cognitive dysfunction in mice. Biochem Biophys Rep. 2017 Jan 05;9:180-186. doi: 10.1016/j.bbrep.2016.12.012. eCollection 2017 Mar. PMID: 28956003; PMCID: PMC5614586.
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Biochem Biophys Rep. 2017 Jan 05;9:180-186. doi: 10.1016/j.bbrep.2016.12.012. eCollection 2017 Mar.

Blood brain barrier permeability of (-)-epigallocatechin gallate, its proliferation-enhancing activity of human neuroblastoma SH-SY5Y cells, and its preventive effect on age-related cognitive dysfunction in mice.

Biochemistry and biophysics reports

Monira Pervin, Keiko Unno, Aimi Nakagawa, Yuu Takahashi, Kazuaki Iguchi, Hiroyuki Yamamoto, Minoru Hoshino, Aya Hara, Akiko Takagaki, Fumio Nanjo, Akira Minami, Shinjiro Imai, Yoriyuki Nakamura

Affiliations

  1. Tea Science Center, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
  2. Department of Neurophysiology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
  3. Food Research Laboratories, Mitsui Norin Co. Ltd., Shizuoka 426-0133, Japan.
  4. Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
  5. School of Bioscience and Biotechnology, Tokyo University of Technology, Tokyo 192-0982, Japan.

PMID: 28956003 PMCID: PMC5614586 DOI: 10.1016/j.bbrep.2016.12.012

Abstract

BACKGROUND: The consumption of green tea catechins (GTCs) suppresses age-related cognitive dysfunction in mice. GTCs are composed of several catechins, of which epigallocatechin gallate (EGCG) is the most abundant, followed by epigallocatechin (EGC). Orally ingested EGCG is hydrolyzed by intestinal biota to EGC and gallic acid (GA). To understand the mechanism of action of GTCs on the brain, their permeability of the blood brain barrier (BBB) as well as their effects on cognitive function in mice and on nerve cell proliferation

METHODS: The BBB permeability of EGCG, EGC and GA was examined using a BBB model kit. SAMP10, a mouse model of brain senescence, was used to test cognitive function

RESULTS: The

CONCLUSION: Cognitive dysfunction in mice is suppressed after ingesting GTCs when a low concentration of EGCG is incorporated into the brain parenchyma via the BBB. Nerve cell proliferation/differentiation was enhanced by a low concentration of EGCG. Furthermore, the additive effect of EGC and GA suggests that EGCG sustains a preventive effect after the hydrolysis to EGC and GA.

Keywords: (−)-epigallocatechin gallate; 8-oxodG, 8-oxodeoxyguanosine; BBB, blood-brain barrier; Blood-brain barrier permeability; Brain plasticity; C, (+)-catechin; Cognitive dysfunction; EC, (−)-epicatechin; EGC, (−)-epigallocatechin; EGCG, (−)-epigallocatechin gallate; GA, gallic acid; GTC, green tea catechin; Green tea catechin; LC-MS/MS, liquid chromatography tandem-mass spectrometry; LPO, lipid peroxidation; MRM, multiple reaction-monitoring; Nerve cell proliferation; SAMP10, senescence-accelerated mouse prone 10.

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