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Arneth B. Activation of CD4+ and CD8+ T-lymphocytes by insulin and GAD in patients with type 1 or 2 diabetes mellitus. Endocr Connect. 2017;6(8):758-765doi: 10.1530/EC-17-0230.
Arneth, B. (2017). Activation of CD4+ and CD8+ T-lymphocytes by insulin and GAD in patients with type 1 or 2 diabetes mellitus. Endocrine connections, 6(8), 758-765. https://doi.org/10.1530/EC-17-0230
Arneth, Borros. "Activation of CD4+ and CD8+ T-lymphocytes by insulin and GAD in patients with type 1 or 2 diabetes mellitus." Endocrine connections vol. 6,8 (2017): 758-765. doi: https://doi.org/10.1530/EC-17-0230
Arneth B. Activation of CD4+ and CD8+ T-lymphocytes by insulin and GAD in patients with type 1 or 2 diabetes mellitus. Endocr Connect. 2017 Nov;6(8):758-765. doi: 10.1530/EC-17-0230. Epub 2017 Oct 06. PMID: 28986401; PMCID: PMC5670273.
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Endocr Connect. 2017 Nov;6(8):758-765. doi: 10.1530/EC-17-0230. Epub 2017 Oct 06.

Activation of CD4+ and CD8+ T-lymphocytes by insulin and GAD in patients with type 1 or 2 diabetes mellitus.

Endocrine connections

Borros Arneth

Affiliations

  1. Institute of Laboratory Medicine and PathobiochemistryMolecular Diagnostics, University Hospital of the Universities of Giessen and Marburg, UKGM, Justus Liebig University, Giessen, Giessen, Germany [email protected].

PMID: 28986401 PMCID: PMC5670273 DOI: 10.1530/EC-17-0230

Abstract

BACKGROUND: The origin of autoimmune disease type 1 diabetes is still unknown.

AIM: This study assessed the activation of CD4+ and CD8+ T-lymphocytes by human insulin and human glutamate decarboxylase (GAD) in patients with type 1 or type 2 diabetes mellitus (DM) and healthy volunteers.

MATERIALS AND METHODS: The expression of CD69, a marker of T-lymphocyte activity, was determined in whole blood samples by flow cytometry after 12 h of incubation with or without insulin or GAD. The analysis included samples from 12 type 1 DM patients, 14 type 2 DM patients and 12 healthy volunteers.

RESULTS: Significant increases in the number of activated CD4+ and CD8+ T-lymphocytes following pre-incubation of whole blood samples with human insulin or GAD were observed in samples from patients with type 1 DM, whereas no activation of these cells was detected in samples from either type 2 DM patients or healthy subjects.

DISCUSSION: These results indicated that latent pre-activation of CD4+ and CD8+ T-lymphocytes in response to insulin or GAD epitopes occurred in type 1 DM patients.

CONCLUSION: These findings suggest that pre-immunization against insulin and/or GAD might be associated with the development of type 1 DM. Alternatively, these results might reflect a non-specific, bystander autoimmune response.

© 2017 The authors.

Keywords: CD4+ T-lymphocytes; CD8+ T-lymphocytes; GAD; autoantibodies; autoimmunity; insulin; type 1 diabetes mellitus; type 2 diabetes mellitus

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