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Kaufmann-Kolle P, Fleer EAM, Kötting J, et al. Liposomal Hexadecylphosphocholine: Characterization and Effects on Adherent Tumor Cells. J Liposome Res. 1994;4(3):1115-1134doi: 10.3109/08982109409018625.
Kaufmann-Kolle, P., Fleer, E. A. M., Kötting, J., Behnert, C., Unger, C., & Eibl, H. (1994). Liposomal Hexadecylphosphocholine: Characterization and Effects on Adherent Tumor Cells. Journal of liposome research, 4(3), 1115-1134. https://doi.org/10.3109/08982109409018625
Kaufmann-Kolle, Petra, et al. "Liposomal Hexadecylphosphocholine: Characterization and Effects on Adherent Tumor Cells." Journal of liposome research vol. 4,3 (1994): 1115-1134. doi: https://doi.org/10.3109/08982109409018625
Kaufmann-Kolle P, Fleer EAM, Kötting J, Behnert C, Unger C, Eibl H. Liposomal Hexadecylphosphocholine: Characterization and Effects on Adherent Tumor Cells. J Liposome Res. 1994;4(3):1115-1134. doi: 10.3109/08982109409018625. PMID: 29052489.
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J Liposome Res. 1994;4(3):1115-1134. doi: 10.3109/08982109409018625.

Liposomal Hexadecylphosphocholine: Characterization and Effects on Adherent Tumor Cells.

Journal of liposome research

Petra Kaufmann-Kolle, Eduard A M Fleer, Jochem Kötting, Christine Behnert, Clemens Unger, Hansjorg Eibl

Affiliations

  1. a Max-Planck-Institut für biophysikalische Chemie, Abt. Membranbiophysik, Göttingen, Germany.
  2. b Medizinische Universitätsklinik, Abt. Hämatologie/Onkologie, Göttingen, Germany.

PMID: 29052489 DOI: 10.3109/08982109409018625

Abstract

We describe the preparation of small unilamellar and multilamellar vesicles from hexadecylphosphocholine, cholesterol and 1,2-dipalmitoyl-sn-glycero-phosphoglycerol in the molar ratio 4/5/1. Particle size and chemical stability of two types of liposomes, small unilamellar vesicles and lyophilized, freshly resuspended multilamellar vesicles were proved to be stable for at least 12 months. Compared to hexadecylphosphocholine in free form, liposomal hexadecylphosphocholine showed remarkably reduced hemolysis which did not change during storage. Fluorescence microscopy showed the uptake of propidium iodide containing hexadecylphosphocholine liposomes by KB and MDA-MB 231 tumor cells. Free propidium iodide was not incorporated into these cells. Although cytotoxicity seemed to be reduced in liposomal preparations, hexadecylphosphocholine liposomes still affected cultured tumor cells to a great extent. In relatively low concentrations they induced shape alteration, smoothing of the cell surface and blebbing.

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