Display options
Cite
Fanuel S, Tabesh S, Sadroddiny E, et al. Analysis of predicted B and T-cell epitopes in Der p 23, allergen from Dermatophagoides pteronyssinus. Bioinformation. 2017;13(9):307-312doi: 10.6026/97320630013307.
Fanuel, S., Tabesh, S., Sadroddiny, E., & Kardar, G. A. (2017). Analysis of predicted B and T-cell epitopes in Der p 23, allergen from Dermatophagoides pteronyssinus. Bioinformation, 13(9), 307-312. https://doi.org/10.6026/97320630013307
Fanuel, Songwe, et al. "Analysis of predicted B and T-cell epitopes in Der p 23, allergen from Dermatophagoides pteronyssinus." Bioinformation vol. 13,9 (2017): 307-312. doi: https://doi.org/10.6026/97320630013307
Fanuel S, Tabesh S, Sadroddiny E, Kardar GA. Analysis of predicted B and T-cell epitopes in Der p 23, allergen from Dermatophagoides pteronyssinus. Bioinformation. 2017 Sep 30;13(9):307-312. doi: 10.6026/97320630013307. eCollection 2017. PMID: 29081610; PMCID: PMC5651224.
Copy Download .nbib Format: NLM
Share it on

Bioinformation. 2017 Sep 30;13(9):307-312. doi: 10.6026/97320630013307. eCollection 2017.

Analysis of predicted B and T-cell epitopes in Der p 23, allergen from Dermatophagoides pteronyssinus.

Bioinformation

Songwe Fanuel, Saeideh Tabesh, Esmaeil Sadroddiny, Gholam Ali Kardar

Affiliations

  1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences-International Campus (IC-TUMS) Tehran, Iran.
  2. Immunology, Asthma & Allergy Research Institute (IAARI), Tehran University of Medical Sciences, Tehran, Iran.
  3. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

PMID: 29081610 PMCID: PMC5651224 DOI: 10.6026/97320630013307

Abstract

House dust mite (HDM) allergy is the leading cause of IgE-mediated hypersensitivity. Therefore identifying potential epitopes in the Dermatophagoide pteronyssinus 23 (Der p 23), a major house dust mite allergen will aid in the development of therapeutic vaccines and diagnostic kits for HDM allergy. Experimental methods of epitope discovery have been widely exploited for the mapping of potential allergens. This study sought to use immunoinformatic methods to analyze the structure of Der p 23 for potential immunoreactive B and T-cell epitopes that could be useful for AIT and allergy diagnosis. We retrieved a Der p 23 allergen sequence from Genbank database and then analyzed it using a combination of web-based sequence analysis tools including the Immune Epitope Database (IEDB), Protparam, BCPREDS, ABCpred, BepiPred, Bcepred among others to predict the physiochemical properties and epitope spectra of the Der p 23 allergen. We then built 3D models of the predicted B-cell epitopes, T cell epitopes and Der p 23 for sequence structure homology analysis. Our results identified peptides 'TRWNEDE', 'TVHPTTTEQPDDK', and 'NDDDPTT' as immunogenic linear B-cell epitopes while 'CPSRFGYFADPKDPH' and 'CPGNTRWNEDEETCT' were found to be the most suitable T-cell epitopes that interacted well with a large number of MHC II alleles. Both epitopes had high population coverage as well as showing a 100% conservancy. These five Der p 23 epitopes are useful for AIT vaccines and HDM allergy diagnosis development.

Keywords: Allergen Immunotherapy; Der p 23; Epitopes; House Dust Mite; immunoinformatics

References

  1. J Immunol. 2014 May 15;192(10):4867-75 - PubMed
  2. Immunome Res. 2006 Jan 21;2:1 - PubMed
  3. Nucleic Acids Res. 2003 Jul 1;31(13):3316-9 - PubMed
  4. J Chem Theory Comput. 2014 Oct 14;10(10):4745-58 - PubMed
  5. Nucleic Acids Res. 2015 Jan;43(Database issue):D405-12 - PubMed
  6. J Comput Chem. 2002 Apr 30;23(6):600-9 - PubMed
  7. Proteins. 2006 Oct 1;65(1):40-8 - PubMed
  8. Curr Opin Allergy Clin Immunol. 2004 Jun;4(3):159-63 - PubMed
  9. Protein Sci. 1993 Sep;2(9):1511-9 - PubMed
  10. Curr Opin Immunol. 2012 Jun;24(3):354-60 - PubMed
  11. In Silico Pharmacol. 2016 Dec;4(1):1 - PubMed
  12. Comput Biol Chem. 2013 Dec;47:149-55 - PubMed
  13. Rev Med Virol. 2009 Mar;19(2):77-96 - PubMed
  14. J Allergy Clin Immunol. 2001 Mar;107(3 Suppl):S406-13 - PubMed
  15. Nucleic Acids Res. 2003 Jul 1;31(13):3784-8 - PubMed
  16. PLoS One. 2012;7(8):e43575 - PubMed
  17. Int Arch Allergy Immunol. 2015;168(3):150-60 - PubMed
  18. Hum Vaccin Immunother. 2012 Oct;8(10):1499-512 - PubMed
  19. Methods Mol Biol. 2000;143:97-129 - PubMed
  20. J Immunol. 2013 Apr 1;190(7):3059-67 - PubMed
  21. Open Biochem J. 2010 Sep 03;4:83-95 - PubMed

Publication Types