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Nagata K, Sawada K, Minomo H, et al. Effects of repeated restraint and blood sampling with needle injection on blood cardiac troponins in rats, dogs, and cynomolgus monkeys. Comp Clin Path. 2017;26(6):1347-1354doi: 10.1007/s00580-017-2539-7.
Nagata, K., Sawada, K., Minomo, H., Sasaki, D., Kazusa, K., & Takamatsu, K. (2017). Effects of repeated restraint and blood sampling with needle injection on blood cardiac troponins in rats, dogs, and cynomolgus monkeys. Comparative clinical pathology, 26(6), 1347-1354. https://doi.org/10.1007/s00580-017-2539-7
Nagata, Keisuke, et al. "Effects of repeated restraint and blood sampling with needle injection on blood cardiac troponins in rats, dogs, and cynomolgus monkeys." Comparative clinical pathology vol. 26,6 (2017): 1347-1354. doi: https://doi.org/10.1007/s00580-017-2539-7
Nagata K, Sawada K, Minomo H, Sasaki D, Kazusa K, Takamatsu K. Effects of repeated restraint and blood sampling with needle injection on blood cardiac troponins in rats, dogs, and cynomolgus monkeys. Comp Clin Path. 2017;26(6):1347-1354. doi: 10.1007/s00580-017-2539-7. Epub 2017 Aug 24. PMID: 29081730; PMCID: PMC5639015.
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Comp Clin Path. 2017;26(6):1347-1354. doi: 10.1007/s00580-017-2539-7. Epub 2017 Aug 24.

Effects of repeated restraint and blood sampling with needle injection on blood cardiac troponins in rats, dogs, and cynomolgus monkeys.

Comparative clinical pathology

Keisuke Nagata, Kazutoshi Sawada, Hirofumi Minomo, Daisuke Sasaki, Katsuyuki Kazusa, Kazuhiko Takamatsu

Affiliations

  1. Drug Safety Research Laboratories, Astellas Pharma Inc., 21 Miyukigaoka, Tsukuba, Ibaraki 305-8585 Japan.
  2. Drug Safety Research Laboratories, Shin Nippon Biomedical Laboratories, Ltd., 2438 Miyanoura, Kagoshima, 891-1394 Japan.

PMID: 29081730 PMCID: PMC5639015 DOI: 10.1007/s00580-017-2539-7

Abstract

While cardiac troponins (cTnT and cTnI) have been used as blood biomarkers of myocardial injury such as myocardial infarction in both humans and animals, their high diagnostic sensitivity inevitably leads to decreased diagnostic specificity. For example, it is difficult to judge whether a slight increase of cardiac troponins in toxicological studies is a treatment-related response or not. Drawing an accurate conclusion requires reliable background data and definitive criteria based on that data. However, no organized efforts in setting such criteria has been reported. Here, we measured blood cTnI and cTnT concentrations in Sprague-Dawley rats, beagle dogs, and cynomolgus monkeys from repeated blood samplings using needle cylinders under restraint up until 24 h after a single oral dose of 0.5 

Keywords: Biomarker; Cardiac troponin; Myocardial injury; cTnI; cTnT

References

  1. Vet Clin Pathol. 2015 Sep;44(3):465-71 - PubMed
  2. Am Heart J. 2011 Jul;162(1):64-73 - PubMed
  3. Toxicol Pathol. 2009 Oct;37(6):754-60 - PubMed
  4. Cancer Chemother Pharmacol. 2001 Oct;48(4):297-304 - PubMed
  5. Circulation. 2011 Nov 22;124(21):2350-4 - PubMed
  6. Regul Toxicol Pharmacol. 2013 Oct;67(1):108-14 - PubMed
  7. J Pharmacol Toxicol Methods. 2013 Jul-Aug;68(1):7-12 - PubMed
  8. Am Heart J. 2009 Jul;158(1):21-9 - PubMed
  9. Lab Anim. 2006 Apr;40(2):153-71 - PubMed

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