Display options
Cite
Fiers M, Hoogenboom J, Brunazzi A, et al. A plant-based chemical genomics screen for the identification of flowering inducers. Plant Methods. 2017;13:78doi: 10.1186/s13007-017-0230-2.
Fiers, M., Hoogenboom, J., Brunazzi, A., Wennekes, T., Angenent, G. C., & Immink, R. G. H. (2017). A plant-based chemical genomics screen for the identification of flowering inducers. Plant methods, 1378. https://doi.org/10.1186/s13007-017-0230-2
Fiers, Martijn, et al. "A plant-based chemical genomics screen for the identification of flowering inducers." Plant methods vol. 13 (2017): 78. doi: https://doi.org/10.1186/s13007-017-0230-2
Fiers M, Hoogenboom J, Brunazzi A, Wennekes T, Angenent GC, Immink RGH. A plant-based chemical genomics screen for the identification of flowering inducers. Plant Methods. 2017 Oct 03;13:78. doi: 10.1186/s13007-017-0230-2. eCollection 2017. PMID: 29026434; PMCID: PMC5627458.
Copy Download .nbib Format: NLM
Share it on

Plant Methods. 2017 Oct 03;13:78. doi: 10.1186/s13007-017-0230-2. eCollection 2017.

A plant-based chemical genomics screen for the identification of flowering inducers.

Plant methods

Martijn Fiers, Jorin Hoogenboom, Alice Brunazzi, Tom Wennekes, Gerco C Angenent, Richard G H Immink

Affiliations

  1. Bioscience, Wageningen University and Research, 6700 AP Wageningen, The Netherlands.
  2. Laboratory of Molecular Biology, Wageningen University and Research, 6708 PB Wageningen, The Netherlands.
  3. Laboratory of Organic Chemistry, Wageningen University and Research, 6708 WE Wageningen, The Netherlands.
  4. Department of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CH Utrecht, The Netherlands.

PMID: 29026434 PMCID: PMC5627458 DOI: 10.1186/s13007-017-0230-2

Abstract

BACKGROUND: Floral timing is a carefully regulated process, in which the plant determines the optimal moment to switch from the vegetative to reproductive phase. While there are numerous genes known that control flowering time, little information is available on chemical compounds that are able to influence this process. We aimed to discover novel compounds that are able to induce flowering in the model plant Arabidopsis. For this purpose we developed a plant-based screening platform that can be used in a chemical genomics study.

RESULTS: Here we describe the set-up of the screening platform and various issues and pitfalls that need to be addressed in order to perform a chemical genomics screening on Arabidopsis plantlets. We describe the choice for a molecular marker, in combination with a sensitive reporter that's active in plants and is sufficiently sensitive for detection. In this particular screen, the firefly Luciferase marker was used, fused to the regulatory sequences of the floral meristem identity gene

CONCLUSIONS: In this study a chemical genomics screening platform was developed to discover compounds that can induce flowering in Arabidopsis. This platform was used successfully, to identify a compound that can speed-up flowering in Arabidopsis.

Keywords: APETALA1; Arabidopsis; Chemical genomics; Flowering; Luciferase; Salicylic acid

References

  1. Science. 2009 May 22;324(5930):1068-71 - PubMed
  2. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):12132-7 - PubMed
  3. Cell Rep. 2012 Jun 28;1(6):639-47 - PubMed
  4. Chem Biol. 2012 Aug 24;19(8):1060-72 - PubMed
  5. Development. 2011 Oct;138(19):4117-29 - PubMed
  6. Nucleic Acids Res. 2016 Jan 4;44(D1):D1167-71 - PubMed
  7. Plant J. 1998 Dec;16(6):735-43 - PubMed
  8. Arabidopsis Book. 2011;9:e0156 - PubMed
  9. Development. 1997 Feb;124(3):645-54 - PubMed
  10. Bioorg Med Chem. 2008 Dec 1;16(23):10043-8 - PubMed
  11. Front Plant Sci. 2015 Mar 19;6:174 - PubMed
  12. PLoS One. 2015 Feb 26;10(2):e0116973 - PubMed
  13. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3585-90 - PubMed
  14. New Phytol. 2010 Jan;185(1):15-26 - PubMed
  15. Front Plant Sci. 2015 Jan 26;5:804 - PubMed
  16. Plant Cell. 1991 Oct;3(10):1085-1094 - PubMed
  17. Nature. 1992 Nov 19;360(6401):273-7 - PubMed
  18. Trends Plant Sci. 2010 Feb;15(2):81-8 - PubMed
  19. Int J Dev Biol. 2005;49(5-6):585-93 - PubMed
  20. Nature. 2006 May 4;441(7089):91-5 - PubMed
  21. Chem Biol. 2010 Jun 25;17(6):646-57 - PubMed
  22. Science. 2007 May 18;316(5827):1030-3 - PubMed
  23. Mol Plant. 2015 Jul;8(7):983-97 - PubMed
  24. Plant Cell. 2012 Sep;24(9):3795-804 - PubMed
  25. J Exp Bot. 2011 Jun;62(10):3321-38 - PubMed
  26. Plant J. 2004 Jan;37(2):209-17 - PubMed
  27. Plant J. 2010 Jul 1;63(1):60-72 - PubMed
  28. Front Plant Sci. 2015 Apr 08;6:131 - PubMed
  29. Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5854-9 - PubMed

Publication Types