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Kancharla V, Bashir S, Liu JL, et al. Effect of metal surfaces on matrix-assisted laser desorption/ionization analyte peak intensities. Eur J Mass Spectrom (Chichester). 2017;23(5):287-299doi: 10.1177/1469066717712694.
Kancharla, V., Bashir, S., Liu, J. L., Ramirez, O. M., Derrick, P. J., & Beran, K. A. (2017). Effect of metal surfaces on matrix-assisted laser desorption/ionization analyte peak intensities. European journal of mass spectrometry (Chichester, England), 23(5), 287-299. https://doi.org/10.1177/1469066717712694
Kancharla, Vidhyullatha, et al. "Effect of metal surfaces on matrix-assisted laser desorption/ionization analyte peak intensities." European journal of mass spectrometry (Chichester, England) vol. 23,5 (2017): 287-299. doi: https://doi.org/10.1177/1469066717712694
Kancharla V, Bashir S, Liu JL, Ramirez OM, Derrick PJ, Beran KA. Effect of metal surfaces on matrix-assisted laser desorption/ionization analyte peak intensities. Eur J Mass Spectrom (Chichester). 2017 Oct;23(5):287-299. doi: 10.1177/1469066717712694. PMID: 29028384.
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Eur J Mass Spectrom (Chichester). 2017 Oct;23(5):287-299. doi: 10.1177/1469066717712694.

Effect of metal surfaces on matrix-assisted laser desorption/ionization analyte peak intensities.

European journal of mass spectrometry (Chichester, England)

Vidhyullatha Kancharla, Sajid Bashir, Jingbo L Liu, Oscar M Ramirez, Peter J Derrick, Kyle A Beran

Affiliations

  1. 1 Department of Chemistry, Texas A&M University-Kingsville, Kingsville, TX, USA.
  2. 2 Institute of Mass Spectrometry, University of Warwick, Coventry, UK.
  3. 5 Department of Physical Sciences, The University of Texas of the Permian Basin, Odessa, TX, USA.
  4. 3 Department of Physics, University of Auckland, Auckland, New Zealand.
  5. 4 Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand.

PMID: 29028384 DOI: 10.1177/1469066717712694

Abstract

Different metal surfaces in the form of transmission electron microscope grids were examined as support surfaces in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with a view towards enhancement of peptide signal intensity. The observed enhancement between 5-fold and 20-fold relative to the normal stainless steel slide was investigated by applying the thermal desorption model for matrix-assisted laser desorption/ionization. A simple model evaluates the impact that the thermal properties of the metals have on the ion yield of the analyte. It was observed that there was not a direct, or strong, correlation between the thermal properties of the metals and the corresponding ion yield of the peptides. The effects of both fixed and variable laser irradiances versus ion yield were also examined for the respective metals studied. In all cases the use of transmission electron microscope grids required much lower laser irradiances in order to generate similar peak intensities as those observed with a stainless steel surface.

Keywords: Matrix-assisted laser desorption/ionization; metal substrates; peptide enhancement; thermal properties; transmission electron microscope grid

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